Analysis of polyamine biosynthetic- and transport ability of human indigenous Bifidobacterium

Bioscience, Biotechnology, and Biochemistry
Yuta SugiyamaShin Kurihara

Abstract

Bifidobacteria are members of the human intestinal microbiota, being numerically dominant in the colon of infants, and also being prevalent in the large intestine of adults. In this study, we measured the concentrations of major polyamines (putrescine, spermidine, and spermine) in cells and culture supernatant of 13 species of human indigenous Bifidobacterium at growing and stationary phase. Except for Bifidobacterium bifidum and Bifidobacterium gallicum, 11 species contained spermidine and/or spermine when grown in Gifu-anaerobic medium (GAM). However, Bifidobacterium scardovii and Bifidobacterium longum subsp. infantis, which contain spermidine when grown in GAM, did not contain spermidine when grown in polyamine-free 199 medium. Of the tested 13 Bifidobacterium species, 10 species showed polyamine transport ability. Combining polyamine concentration analysis in culture supernatant and in cells, with basic local alignment search tool analysis suggested that novel polyamine transporters are present in human indigenous Bifidobacterium. Put: putrescine; Spd: spermidine; Spm: spermine; GAM: Gifu anaerobic medium; BHI: brain-heart infusion.

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Citations

Feb 8, 2020·Inflammatory Bowel Diseases·Anna NiechcialMarianne R Spalinger
Oct 18, 2020·Nature Reviews. Neuroscience·Brittany D NeedhamSarkis K Mazmanian
Oct 16, 2019·Journal of Pharmaceutical and Biomedical Analysis·Jessica FioriRoberto Gotti
Oct 2, 2021·Letters in Applied Microbiology·Rong YangShunxiang Li

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Methods Mentioned

BETA
GAM
High Performance Liquid Chromatography

Software Mentioned

BLAST
SPSS
GAM

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