Analysis of POU5F1, c-Kit, PLAP, AP2γ and SALL4 in gonocytes of patients with cryptorchidism

Acta histochemica
Rosa María Vigueras-VillaseñorJulio César Rojas-Castañeda

Abstract

Cryptorchidism is a risk factor for the development of testicular germ cell tumors (TGCTs). The most common type of TGCT in cryptorchidism is seminoma. The intratubular germ cell neoplasia unclassified (ITGCNU) is a histological pattern preceding the development of seminomas and non-seminomas. It was suggested that in patients with cryptorchidism, the gonocytes remained undifferentiated with pluripotent abilities expressing proteins like POU domain class 5 transcription factor 1 (POU5F1), tyrosine kinase receptor c-Kit, placental-like alkaline phosphatase (PLAP), the transcription factor AP2γ and sal-like protein 4 (SALL4) that confer to the gonocytes this ability and therefore make them susceptible to develop ITGCNU. The aim of the present study was to determine if the gonocytes of patients with cryptorchidism express POU5F1, c-Kit, PLAP, AP2γ and SALL4 proteins after their differentiation period. Based on this, we evaluated samples of testicular tissue from newborns to 16-year old subjects with or without cryptorchidism in search of POU5F1, c-Kit, PLAP, AP2γ and SALL4 using immunocytochemical method, the results of which were validated by RT-PCR. The results showed that control subjects witnessed a down-regulation in the expr...Continue Reading

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Citations

Jan 19, 2019·Human Genetics·Rossella CannarellaAldo E Calogero
Jul 6, 2019·Asian Journal of Andrology·Li-Hua DongClaus Yding Andersen
Aug 11, 2020·Pediatric Surgery International·Kolja KvistJorgen Thorup
Jun 19, 2018·Frontiers in Endocrinology·Kolja KvistJorgen Thorup
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Daniel Adrian Landero-HuertaMargarita Dolores Chávez-Saldaña
Jan 20, 2021·Journal of Pediatric Surgery·Rosa María Vigueras-VillaseñorJulio César Rojas-Castañeda

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