PMID: 20657157Jul 27, 2010Paper

Analysis of prolactin gene expression and cleaved prolactin variants in the mouse testis and spermatozoa

The Journal of Reproduction and Development
Michiyo IshidaToshio Harigaya

Abstract

Prolactin (PRL) has long been known to be a hormone responsible for mammary gland development and lactation in females, whereas its role in males is still unclear. Thus, we investigated male mouse (m) PRL protein and mRNA expression in spermatozoa at various differentiation stages in the testes. Quantitative RT-PCR and in situ hybridization detected the expression of PRL not only in Leydig cells but also in germ cells, in particular in spermatogonia. The nucleotide sequence of testis PRL mRNA was the same as that in the pituitary. The mPRL was detected in Leydig cells and in round and elongated spermatids of the testes by immunohistochemistry. Immunoblotting detected 2 forms of mPRL in the testes, one form was 23-kDa PRL, and the other form was smaller than full-length PRL. Based on these results, we focused on N-terminal cleaved PRL to determine its involvement in spermatogenesis. Immunohistochemistry using two sets of antibodies, one that recognized full-length PRL and N-terminal cleaved PRL and another that recognized full-length PRL and C-terminal cleaved PRL, suggested that intact PRL was localized in the nucleus of round spermatids, while N-terminal cleaved PRL variants were localized in the Golgi apparatus of the sperrma...Continue Reading

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Citations

May 29, 2012·General and Comparative Endocrinology·Kazuki MaedaSakae Takeuchi
Jul 13, 2011·General and Comparative Endocrinology·Shusuke TaniuchiSakae Takeuchi
Nov 7, 2015·Reviews in Endocrine & Metabolic Disorders·Giulia RastrelliMario Maggi
Dec 3, 2014·General and Comparative Endocrinology·Steve HarveyCarlos Arámburo
Feb 17, 2015·General and Comparative Endocrinology·Guixian BuYajun Wang
May 9, 2012·Hormones and Behavior·Karin Viana WeissheimerGilberto Luiz Sanvitto
Apr 4, 2014·Molecular Endocrinology·Robert J Marano, Nira Ben-Jonathan
Dec 15, 2015·Journal of Biological Rhythms·Eric L Bittman
Aug 28, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jakob TriebelCarmen Clapp
Apr 25, 2016·The Journal of Poultry Science·Gen HiyamaTomohiro Sasanami

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