Analysis of protein coatings on gold nanoparticles by XPS and liquid-based particle sizing techniques

Biointerphases
Natalie A BelseyCaterina Minelli

Abstract

The precise use of nanoparticles in technological applications requires control over their surface properties. This implies the ability to quantitatively describe, for example, molecular coatings in terms of their thickness, areal mass, or number of molecules. Here, the authors describe two different approaches to the measurement of these parameters by using gold nanoparticles ranging in diameter from 10 to 80 nm and coated with three different proteins: immunoglobulin G, bovine serum albumin, and a peptide. One approach utilizes ultraviolet-visible spectroscopy, dynamic light scattering, and differential centrifugal sedimentation to measure the protein shell refractive indices and thicknesses, from which the number of molecules in the protein shell can be derived. The other approach employs x-ray photoelectron spectroscopy to measure the thickness of the dry molecular coatings and also to derive the number of molecules in the protein shell. The authors demonstrate that the two approaches, although very different, produce consistent measurement results. This finding is important to extend the quantitative analysis of nanoparticle molecular coatings to a wide range of materials.

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Citations

Feb 13, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Aneta SikoraCaterina Minelli
Apr 19, 2016·Surface and Interface Analysis : SIA·David J H CantAlexander G Shard
Aug 31, 2016·Microscopy Today·Donald R BaerDavid G Castner
Apr 3, 2016·Analytical and Bioanalytical Chemistry·Pablo Darío QuevedoUte Resch-Genger
Nov 8, 2016·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Natalie A BelseyAlexander G Shard
May 6, 2019·Electrophoresis·Siyao Liu, Michael Lämmerhofer
Jun 4, 2020·Biointerphases·Domenik ProzellerKatharina Landfester
Dec 20, 2020·International Journal of Biological Macromolecules·Turkan Kopac
May 13, 2021·Annual Review of Analytical Chemistry·Tobias Weidner, David G Castner
Jun 12, 2018·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·C J PowellD G Castner
Feb 1, 2017·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·C J PowellD G Castner
Mar 8, 2019·Bioconjugate Chemistry·Guadalupe RuizJeremy D Driskell
Jan 18, 2017·Bioconjugate Chemistry·Elena ColangeloRaphaël Lévy
Apr 2, 2019·Analytical Chemistry·Michael J EllerEmile A Schweikert
Jan 14, 2021·The Journal of Physical Chemistry. B·Arghyadeep BasuLev Chuntonov

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