Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique

Journal of Virological Methods
Dongbo SunLi Feng

Abstract

Recent outbreaks of porcine epidemic diarrhea virus (PEDV) have caused widespread concern. The identification of proteins associated with PEDV infection might provide insight into PEDV pathogenesis and facilitate the development of novel antiviral strategies. We analyzed the differential protein profile of PEDV-infected Vero E6 cells using mass spectrometry and an isobaric tag for relative and absolute quantification. A total of 126 proteins were identified that were differentially expressed between the PEDV-infected and mock-infected groups (P<0.05, quantitative ratio ≥1.2), among which the expression of 58 proteins was up-regulated and that of 68 proteins was down-regulated in the PEDV-infected Vero E6 cells, involving in integrin β2/β3, cystatin-C. The Gene Ontology analysis indicated that the molecular function of the differentially expressed proteins (DEPs) was primarily related to binding and catalytic activity, and that the biological functions in which the DEPs are involved included metabolism, organismal systems, cellular processes, genetic information processing, environmental information processing, and diseases. Among the disease-related functions, certain anti-viral pathways and proteins, such as the RIG-I-like rec...Continue Reading

References

Jan 1, 1978·Archives of Virology·M B Pensaert, P de Bouck
Nov 1, 1991·Antimicrobial Agents and Chemotherapy·A R Collins, A Grubb
Jan 1, 1986·Journal of Cellular Biochemistry·B D KorantV Turk
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Nov 6, 2004·FEBS Letters·Tetsuya MizutaniShigeru Morikawa
May 27, 2005·Biochimica Et Biophysica Acta·Tetsuya MizutaniShigeru Morikawa
Jun 28, 2005·Nucleic Acids Research·E QuevillonR Lopez
Nov 9, 2007·Trends in Microbiology·Phoebe L Stewart, Glen R Nemerow
Mar 4, 2008·Journal of Proteome Research·Hubert HondermarckXuefen Le Bourhis
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Jul 25, 2009·Emerging Infectious Diseases·Suphasawatt PuranavejaRoongroje Thanawongnuwech
Jun 15, 2010·Archives of Virology·Jianfei ChenLi Feng
Jul 31, 2010·Mediators of Inflammation·Shin-Ichi YokotaNobuhiro Fujii
Aug 19, 2010·Journal of Proteome Research·Emily BojaHenry Rodriguez
Sep 10, 2010·Journal of Virology·Takayuki HishikiKunitada Shimotohno
Oct 1, 2010·Biotechnology Letters·Xiaofeng RenYong-Suk Jang
May 27, 2011·Journal of Virology·Kazuya ShiratoFumihiro Taguchi
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Guiqing PengFang Li
Nov 15, 2011·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Dec 14, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jifeng YuanChi B Ching
Mar 27, 2012·Journal of Proteome Research·Ying HeJianping Xie
Jul 21, 2012·Viruses·Sandrine BelouzardGary R Whittaker
Jul 31, 2012·Emerging Infectious Diseases·Wentao LiQigai He
Oct 31, 2012·Virus Research·Youngnam Kim, Changhee Lee
Dec 4, 2012·Viruses·Helena J Maier, Paul Britton
Feb 9, 2013·Journal of Innate Immunity·Xiaonan Dong, Beth Levine
Apr 23, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Qinghe ZhuDongbo Sun
May 31, 2013·The Journal of Biological Chemistry·Irshad Ali SheikhKazi Mirajul Hoque
Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Danyelle N Martin, Susan L Uprichard
Aug 22, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Gregory W StevensonKyoungjin J Yoon
Oct 12, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Donghua GuoDongbo Sun
Jan 15, 2014·JAK-STAT·Aaron F HeneghanKenneth A Kudsk

❮ Previous
Next ❯

Citations

Jul 12, 2017·Viruses·Yingying CongFulvio Reggiori
Jul 7, 2020·Endocrine Research·Paul J DavisShaker A Mousa
Jun 24, 2020·Frontiers in Microbiology·Changlin WangPinghuang Liu
May 21, 2020·International Journal of Molecular Sciences·Valentino ClementeMartina Bazzaro
Dec 1, 2020·Autophagy·Marie-Angela DomdomBaharia Mograbi
Sep 21, 2020·Developmental and Comparative Immunology·Chongyang LiHongjuan Cui

❮ Previous
Next ❯

Methods Mentioned

BETA
IECs

Software Mentioned

Image Lab
Proteome Discoverer
Blast2GO
MASCOT
Excel

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.