Analysis of protein glycation in human fingernail clippings with near-infrared (NIR) spectroscopy as an alternative technique for the diagnosis of diabetes mellitus.

Clinical Chemistry and Laboratory Medicine : CCLM
Tinne MonteyneJoris R Delanghe

Abstract

Glycated keratin allows the monitoring of average tissue glucose exposure over previous weeks. In the present study, we wanted to explore if near-infrared (NIR) spectroscopy could be used as a non-invasive diagnostic tool for assessing glycation in diabetes mellitus. A total of 52 patients with diabetes mellitus and 107 healthy subjects were enrolled in this study. A limited number (n=21) of nails of healthy subjects were glycated in vitro with 0.278 mol/L, 0.556 mol/L and 0.833 mol/L glucose solution to study the effect of glucose on the nail spectrum. Consequently, the nail clippings of the patients were analyzed using a Thermo Fisher Antaris II Near-IR Analyzer Spectrometer and near infrared (NIR) chemical imaging. Spectral classification (patients with diabetes mellitus vs. healthy subjects) was performed using partial least square discriminant analysis (PLS-DA). In vitro glycation resulted in peak sharpening between 4300 and 4400 cm-1 and spectral variations at 5270 cm-1 and between 6600 and 7500 cm-1. Similar regions encountered spectral deviations during analysis of the patients' nails. Optimization of the spectral collection parameters was necessary in order to distinguish a large dataset. Spectra had to be collected at...Continue Reading

References

Mar 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·E Bakan, N Bakan
May 22, 2008·The International Journal of Lower Extremity Wounds·Michael Edmonds
Jun 9, 2009·Diabetes Care·UNKNOWN International Expert Committee
Sep 12, 2009·Journal of the European Academy of Dermatology and Venereology : JEADV·S YaemsiriK He
Jul 9, 2010·Lancet·Jean Claude N MbanyaSostanie T Enoru
Dec 7, 2010·Acta Clinica Belgica·K Janssen, J Delanghe
Dec 21, 2010·International Journal of Pharmaceutics·T De BeerC Vervaet
Jan 13, 2012·The Journal of Clinical Endocrinology and Metabolism·William H Herman, Robert M Cohen
Sep 10, 2013·Biotechnology Progress·Laurent HansenRim Daoussi
Nov 2, 2013·Tropical Medicine & International Health : TM & IH·Antoine Sadiki KishabongoJoris R Delanghe
Aug 10, 2017·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Sonak D PastakiaLydia Fischer

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Citations

Jun 16, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Stéphane Jaisson, Philippe Gillery
Apr 3, 2019·International Journal of Molecular Sciences·Tijl VermassenJoris Delanghe
Jul 22, 2018·Acta Clinica Belgica·Justin C CikomolaJoris R Delanghe
Oct 13, 2020·Critical Reviews in Clinical Laboratory Sciences·Sander De BruyneJoris R Delanghe
Jun 23, 2021·Current Opinion in Clinical Nutrition and Metabolic Care·Stéphane Jaisson, Philippe Gillery
May 28, 2021·Applied Spectroscopy·James M CameronMatthew J Baker
Nov 3, 2021·Critical Reviews in Clinical Laboratory Sciences·Sarahi Jaramillo OrtizFrédéric J Tessier

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