Analysis of protein interactions on protein arrays by a wavelength interrogation-based surface plasmon resonance biosensor

Proteomics
Jong Seol YukKwon-Soo Ha

Abstract

We have investigated whether surface plasmon resonance (SPR) sensors based on the wavelength interrogation are able to analyze protein interactions on protein arrays. The spectral SPR sensor was self-constructed and its detection limit, expressed as the minimal refractive index variation, was calculated to be 6.6x10(-5) with the signal fluctuation of 1.0x10(-5). The protein array surface was modified by a mixed thiol monolayer to immobilize proteins. Protein arrays were analyzed by the line-scanning mode of the SPR sensor, which scanned every 100 microm along the central line of array spots and the scanned results were presented by color spectra from blue to red. Glutathione S-transferase (GST)-rac1 caused a concentration-dependent increase of SPR wavelength shift on protein arrays. The surface structure of the protein arrays was analyzed by atomic force microscopy. Specific interactions of antigens with antibodies were analyzed on the protein arrays by using three antibodies and eight proteins. These results suggest that the wavelength interrogation-based SPR sensor can be used as the biosensor for the high-throughput analysis of protein interactions on protein arrays.

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Citations

Dec 16, 2006·Analytical and Bioanalytical Chemistry·Denis HabauzitBenoit Roig
Apr 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Nader PourmandRonald W Davis
Jul 31, 2009·IET Nanobiotechnology·S PaulA K Ray
Oct 8, 2005·Expert Review of Proteomics·Josef G HeuerBrian T Edmonds
Oct 22, 2008·Applied Optics·Le LiuJihua Guo
Sep 12, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Stella SunJohn M Luk
Aug 2, 2006·Molecular BioSystems·Mahesh UttamchandaniShao Q Yao

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