Analysis of puff dynamics in oocytes: interdependence of puff amplitude and interpuff interval

Biophysical Journal
Daniel FraimanSilvina Ponce Dawson

Abstract

Puffs are localized Ca(2+) signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP(3)). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca(2+) from the endoplasmic reticulum through the coordinated opening of IP(3) receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca(2+) that is liberated during puffs. We construct a stochastic model for a cluster of IP(3) receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds.

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Citations

Apr 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ian F Smith, Ian Parker
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Geneviève DupontJames W Putney
Jun 14, 2012·PLoS Computational Biology·Sten RüdigerJian-Wei Shuai
Feb 23, 2010·PloS One·Guillermo Solovey, Silvina Ponce Dawson
Apr 30, 2014·PloS One·Estefanía PiegariSilvina Ponce Dawson
Oct 2, 2009·Chaos·D SwaminathanP Jung
Nov 2, 2011·Biochimica Et Biophysica Acta·Kevin ThurleyMartin Falcke
Jan 26, 2010·Cell Calcium·Luciana BrunoSilvina Ponce Dawson
Apr 8, 2009·Journal of Theoretical Biology·Erin R HigginsMartin Falcke
Oct 31, 2006·Cell Calcium·John G McCarronThomas C Muir
Sep 10, 2013·Biophysical Journal·Pengxing CaoJames Sneyd
May 27, 2014·Frontiers in Molecular Neuroscience·Kevin B Clark
May 21, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·J W ShuaiS Rüdiger
Jan 15, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Ana CalabreseSilvina Ponce Dawson
Jan 31, 2014·Physical Biology·Daniel Fraiman, Silvina Ponce Dawson
Apr 2, 2019·Physical Review Letters·V VoorsluijsG Dupont
Aug 17, 2019·Frontiers in Physiology·Estefanía PiegariSilvina Ponce Dawson

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