PMID: 3757932Jan 1, 1986Paper

Analysis of pulmonary phospholipid compartments in the unanesthetized rat during prolonged periods of hyperpnea

Experimental Lung Research
J H PowerT E Nicholas

Abstract

We exposed rats to 4% CO2-10% O2-86% N2 for 24 h before infusing with 20 microCi kg-1 (methyl-3H)choline chloride. They were then exposed for periods up to 48 h, at which times their lungs were degassed and lavaged. The lavage fluid was divided into a tubular myelin-rich (PLalv-1) and a tubular myelin-poor (PLalv-2) fraction. Lamellar body (lb) and microsomal (m) fractions were prepared from the lung tissue and the amount of phospholipid (PL) was determined in each of the 4 fractions. Specific activity (sp.act.) curves were constructed for both control and hyperpneic groups. Exposure to the gas doubled both tidal volume and frequency of breathing. Total PLalv, PLlb and PLm were all markedly elevated, which, when taken in conjunction with the changes in sp.act. in these fractions, suggests that the rate of surfactant PL synthesis was increased within 24 h. The shapes of the sp.act. curves suggest precursor-product relationships between PLlb and PLalv-1 and between PLalv-1 and PLalv-2. However, when we applied analysis based on the Zilversmit steady-state equation, instead of the expected straight line, we found a marked clockwise hysteresis that did not return to the origin. Whereas this may reflect PLalv being supplied from 2 t...Continue Reading

References

Nov 1, 1972·The Tohoku Journal of Experimental Medicine·N ToshimaK Ono
Sep 1, 1967·The American Journal of Physiology·D F TierneyH J Trahan
Jul 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·T E Nicholas, H A Barr
Apr 1, 1983·Experimental Lung Research·J W SpaldingG E Hook
Sep 1, 1984·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·M E Jones, T E Nicholas
Apr 1, 1983·Respiration Physiology·T E Nicholas, H A Barr
Jan 7, 1983·Biochimica Et Biophysica Acta·M W MagoonJ A Clements
Aug 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·S L YoungG W Brumley
Sep 1, 1982·Respiration Physiology·T E NicholasH A Barr
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Nov 1, 1986·International Journal of Bio-medical Computing·M E JonesH A Barr
Nov 1, 1987·International Journal of Bio-medical Computing·M E JonesT E Nicholas
Apr 1, 1989·Respiration Physiology·L BrondelJ P Besombes
Jan 1, 1992·Respiration Physiology·H E Ward, T E Nicholas
May 23, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S Orgeig, C B Daniels
Jun 5, 2002·American Journal of Respiratory and Critical Care Medicine·Kate G DavidsonIan R Doyle
Sep 1, 1990·American Journal of Respiratory Cell and Molecular Biology·T E NicholasH A Barr
Jan 1, 1993·American Journal of Respiratory Cell and Molecular Biology·J H PowerT E Nicholas
Jun 23, 2009·Respiratory Physiology & Neurobiology·Dani-Louise DixonAndrew D Bersten
Nov 14, 1998·Biochimica Et Biophysica Acta·R J Mason, D R Voelker
Oct 7, 2000·Respirology : Official Journal of the Asian Pacific Society of Respirology·I R DoyleT E Nicholas
Jan 1, 1988·Annals of the New York Academy of Sciences·R A Gibson, E J McMurchie
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·K G DavidsonI R Doyle
Aug 19, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Randolph H HastingsLeonard J Deftos

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