Analysis of QTL DM4.1 for Downy Mildew Resistance in Cucumber Reveals Multiple subQTL: A Novel RLK as Candidate Gene for the Most Important subQTL

Frontiers in Plant Science
Jeroen A BergHenk J Schouten

Abstract

One of the biggest problems in cucumber cultivation is cucurbit downy mildew (DM), caused by the obligate biotroph Pseudoperonospora cubensis. Whereas DM in cucumber was previously efficiently controlled by the dm-1 gene from Indian cucumber accession PI 197087, this resistance was broken by new DM strains, prompting the search for novel sources of resistance. A promising source of resistance is the wild cucumber accession PI 197088. It was previously shown that DM resistance in this genotype inherits polygenically. In this paper, we put the focus on one of the QTL, DM4.1 that is located on chromosome 4. QTL DM4.1 was shown to consist of three subQTL: DM4.1.1 affected pathogen-induced necrosis, DM4.1.2 was shown to have an additive effect on sporulation, and DM4.1.3 had a recessive effect on chlorosis as well as an effect on sporulation. Near-isogenic lines (NILs) were produced by introgressing the subQTLs into a susceptible cucumber line (HS279) with good horticultural traits. Transcriptomic analysis revealed that many genes in general, and defense pathway genes in particular, were differentially expressed in NIL DM4.1.1/.2 compared to NIL DM4.1.3 and the susceptible parent HS279. This indicates that the resistance from subQTL...Continue Reading

References

Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·X WangM Lawton
Jan 1, 1997·The Plant Journal : for Cell and Molecular Biology·C FeuilletB Keller
Sep 7, 2001·Current Opinion in Cell Biology·B D Kohorn
Feb 28, 2002·Genetical Research·D L RemingtonM D Purugganan
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Olivier VoinnetDavid Baulcombe
May 2, 2003·Bioinformatics·Karl W BromanGary A Churchill
Jun 11, 2003·The Plant Journal : for Cell and Molecular Biology·Eddy P RisseeuwWilliam L Crosby
Jun 14, 2003·Plant Physiology·Shin Han Shiu, Anthony B Bleecker
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Sep 24, 2009·Journal of Experimental Botany·Klaas Bouwmeester, Francine Govers
Nov 3, 2009·Nature Genetics·Sanwen HuangSonggang Li
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Feb 1, 2011·The Plant Cell·Bart P H J ThommaMatthieu H A J Joosten
Mar 2, 2011·Molecular Plant Pathology·Elizabeth A SavoryBrad Day
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jul 9, 2014·Annual Review of Phytopathology·Chris C N van Schie, Frank L W Takken
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Dec 23, 2014·Nature Genetics·Weiliang ZuoMingliang Xu
Jul 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Severine HurniBeat Keller
Oct 29, 2015·Journal of Experimental Botany·Bruce D Kohorn
May 6, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yuhui WangYiqun Weng
Sep 22, 2016·BMC Bioinformatics·Tyler W H Backman, Thomas Girke
Mar 18, 2017·The Plant Cell·Dingzhong TangJian-Min Zhou
Nov 22, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yuhui WangYiqun Weng

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRA056480
PRJNA544259
MK936607

Methods Mentioned

BETA
genotyping
PCR
RNAseq
PCA
ubiquitination

Software Mentioned

InterProScan
SnpEff
HTSeq
package
BLAST
BLASTp
R
Bedtools
Integrative Genomics Viewer ( IGV
Samtools mpileup

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.