PMID: 9634306Jun 20, 1998Paper

Analysis of quinolone resistance mechanisms in Neisseria gonorrhoeae isolates in vitro

Sexually Transmitted Infections
M TanakaJ Kumazawa

Abstract

Gonococcal fluoroquinolone resistance is now a significant problem in Japan. We generated gonococcal mutants resistant to norfloxacin in vitro from norfloxacin sensitive isolates and analysed the contribution of three known mechanisms of quinolone resistance in Neisseria gonorrhoeae. Three clinical isolates of N gonorrhoeae susceptible to norfloxacin were exposed to increasing concentrations of norfloxacin. To identify mutations in the gyrA and parC genes of the gonococcal mutants, the quinolone resistance determining regions of the gyrA and parC genes were polymerase chain reaction (PCR) amplified and the PCR products were directly sequenced. Norfloxacin accumulation in the gonococcal cells was also measured. The MICs of norfloxacin for three variants containing a single GyrA mutation were 16-fold higher than that for their parent isolates. A variant showing reduced norfloxacin accumulation in the cells, without mutations in the GyrA or ParC proteins, was also less sensitive to norfloxacin, with a 16-fold increase in the MIC, compared with the parent strain. The MIC of norfloxacin for a variant which contained a single GyrA mutation with reduced norfloxacin accumulation in the cells was 128-fold higher than for the parent stra...Continue Reading

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Citations

Oct 19, 2002·International Journal of Antimicrobial Agents·Prashini MoodleyA Willem Sturm
Dec 22, 1999·Microbes and Infection·Y T van Duynhoven
Nov 26, 2010·Antimicrobial Agents and Chemotherapy·Vanessa G AllenRoberto G Melano
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Kai Man KamMay May Cheung
Mar 23, 2011·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·M M RodriguesA P Fernandes
Jun 25, 2008·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Kazuyoshi OsakaShoichi Onodera
Apr 29, 2006·International Journal of Urology : Official Journal of the Japanese Urological Association·Katsumi ShigemuraMasato Fujisawa
Jun 21, 2005·Infectious Disease Clinics of North America·Khalil G GhanemJonathan M Zenilman
Nov 20, 2002·Sexually Transmitted Diseases·David L TreesPeter H Kilmarx
Feb 22, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S D MehtaJ M Zenilman
Jan 8, 2004·Journal of Women's Health·Barbara D ReedJulie Horrocks
Apr 23, 2004·Expert Opinion on Pharmacotherapy·Michael Dan
Dec 23, 2008·American Journal of Respiratory Cell and Molecular Biology·Tsutomu ShinoharaYoshimi Shibata
Sep 17, 2011·Internal Medicine·Ai SuzakiSatoshi Hayakawa
Oct 13, 2006·Current Opinion in Infectious Diseases·J A Dillon, F Pagotto
Apr 10, 2019·BMC Microbiology·Mary Wandia KivataJames D Mancuso
Jun 30, 2000·Biochemical Pharmacology·F Van BambekeP M Tulkens

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