Jan 3, 1977

Analysis of rabbit brain polysomal poly (A+) mRNA by DNA excess hybridization

Biochimica Et Biophysica Acta
J J Heikkila, I R Brown

Abstract

DNA sequence representation in rabbit brain mRNA was examined by DNA excess hybridization. purified polysomal poly (A+) mRNA was labeled in vitro with [3H] dimethyl sulfate and reacted at DNA: RNA ratios of 5000 : 1 and 30 000 : 1. Poly(A+) mRNA hybridizied mainly to nonrepeated DNA with a smaller kinetic component which was complementary to repeated DNA sequences. The latter component was not due to nuclear or ribosomal RNA contamination. Poly(A-) RNA purified from brain polysomes hybridized to excess DNA as a single repeated component. Thermal denaturation profiles of the RNA - DNA hybrids indicated a high degree of fidelity in base pairing.

  • References17
  • Citations10

Mentioned in this Paper

Polyribosomes
Adenine Polynucleotides
Nucleic Acid Renaturation
RNA Denaturation
Chinchilla Rabbits
Brain Chemistry
Genomic Hybridization
15S RNA
Poly(A) Tail
Metazoa

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