PMID: 15348048Sep 7, 2004Paper

Analysis of rat plasma proteins desorbed from gold and methyl- and hydroxyl-terminated alkane thiols on gold surfaces

Journal of Materials Science. Materials in Medicine
M KälltorpP Tengvall

Abstract

It is believed that adsorbed blood or plasma components, such as water, peptides, carbohydrates and proteins, determine key events in the concomitant inflammatory tissue response close to implants. The aim of the present study was to develop a procedure for the collection and analysis of minor amounts of proteins bound to solid metal implant surfaces. The combination of a sodium dodecyl sulfate washing method coupled with a polyacylamide gel electrophoretic protein separation technique (SDS-PAGE), Western blot and image analysis enabled the desorption, identification and semiquantification of specific proteins. The analyzed proteins were albumin, immunoglobulin G, fibrinogen and fibronectin. Concentration procedures of proteins were not required with this method despite the small area of the test surfaces. The plasma proteins were adsorbed to pure gold and hydroxylated and methylated gold surfaces, which elicit different tissue responses in vivo and plasma protein adsorption patterns in vitro. The image analysis revealed that the pure gold surfaces adsorbed the largest amount of total and specific proteins. This is in accordance with previous ellipsometry/antibody experiments in vitro. Further, the principles described for the ...Continue Reading

Citations

Jul 2, 2010·Journal of the Royal Society, Interface·Anders PalmquistPeter Thomsen
Oct 1, 2003·Journal of Biomedical Materials Research. Part a·Ma Cristina L MartinsMário A Barbosa
Jul 28, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Leila SafazadehBrad J Berron
Jun 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Abshar HasanLalit M Pandey
Jul 18, 2001·Journal of the American Chemical Society·S PathakM E Thompson
Sep 21, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Jian QinMamoun Muhammed

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