Analysis of RecA-independent recombination events between short direct repeats related to a genomic island and to a plasmid in Escherichia coli K12

PeerJ
María F Azpiroz, Magela Laviña

Abstract

RecA-independent recombination events between short direct repeats, leading to deletion of the intervening sequences, were found to occur in two genetic models in the Escherichia coli K12 background. The first model was a small E. coli genomic island which had been shown to be mobile in its strain of origin and, when cloned, also in the E. coli K12 context. However, it did not encode a site-specific recombinase as mobile genomic islands usually do. It was then deduced that the host cells should provide the recombination function. This latter was searched for by means of a PCR approach to detect the island excision in E. coli K12 mutants affected in a number of recombination functions, including the 16 E. coli K12 site-specific recombinases, the RecET system, and multiple proteins that participate in the RecA-dependent pathways of homologous recombination. None of these appeared to be involved in the island excision. The second model, analyzed in a RecA deficient context, was a plasmid construction containing a short direct repeat proceeding from Saccharomyces cerevisiae, which flanked the cat gene. The excision of this gene by recombination of the DNA repeats was confirmed by PCR and through the detection, recovery and characte...Continue Reading

References

Nov 1, 1990·Journal of Bacteriology·M LaviñaF Moreno
Nov 1, 1994·Molecular & General Genetics : MGG·S T LovettP T Drapkin
Jan 1, 1994·Molecular Microbiology·R KolodnerC Luisi-DeLuca
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M Bzymek, S T Lovett
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·María Eloisa PoeyMagela Laviña
Jan 31, 2009·FEMS Microbiology Reviews·Mario JuhasDerrick W Crook
Jan 27, 2011·Nature Communications·Xiaoxue WangThomas K Wood
Apr 13, 2013·Antimicrobial Agents and Chemotherapy·Claudio PalmieriPietro E Varaldo
Jul 15, 2015·PloS One·Anthony W KingstonElisabeth A Raleigh
Jan 18, 2017·Journal of Bacteriology·Anthony W KingstonElisabeth A Raleigh

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Citations

Mar 12, 2020·Microbial Cell Factories·Tingting DingYi-Xin Huo

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Methods Mentioned

BETA
PCR
genetic exchange

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