PMID: 11328378May 1, 2001Paper

Analysis of recombinant mycobacteria as T helper type 1 vaccines in an allergy challenge model

Immunology
R JanssenJ Thole

Abstract

The potential for development of mycobacteria as T helper type 1 (Th1) vaccines capable of induction of Th1 responses to recombinant antigens was explored in a model system based on an immunodominant peptide from house dust mite. Different recombinant mycobacterial preparations were compared for their ability to induce a Th1 response to the peptidea. It was found that mycobacterial viability was not a prerequisite for Th1 immunogenicity. A dominant interferon-gamma (IFN-gamma) response to peptide was observed in splenocytes from C57BL/6J mice immunized with live or heat-killed preparations of recombinant Mycobacterium vaccae or with live attenuated bacillus Calmette-Guèrin (BCG) vaccine expressing the antigen. Interleukin-5 (IL-5), a marker of a Th2 response, was detected only in mice receiving live M. vaccae. A similar pattern was observed in BALB/b mice, although the magnitude of the IFN-gamma response was much lower. Control and immunized mice were subsequently exposed to allergen using a Th2-inducing challenge protocol. A significant shift from a Th2 to a Th1 response was observed in immunized mice, as judged by cytokine expression by splenocytes and by subclass of circulating antibody. The effect was seen in three inbred m...Continue Reading

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Citations

Apr 24, 2007·Current Allergy and Asthma Reports·Charles C ObiharaNulda Beyers
Aug 17, 2002·Current Opinion in Immunology·Klaus J Erb, Gisela Wohlleben
Jun 23, 2005·International Archives of Allergy and Immunology·L García-MarcosR Busquets Monge
Sep 8, 2004·Treatments in Respiratory Medicine·Leif Bjermer, Zuzana Diamant
Aug 22, 2009·Redox Report : Communications in Free Radical Research·Kenneth J RodgersUlf T Brunk
Jun 6, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A G Jarnicki, W R Thomas
Mar 5, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C HubeauB Vargaftig
Jan 19, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C C Obihara, P G Bardin
May 6, 2006·The Journal of Allergy and Clinical Immunology·Anthony A Horner
Jul 19, 2003·International Journal of Medical Microbiology : IJMM·Claudia Trujillo, Klaus J Erb

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