PMID: 8443340Feb 1, 1993Paper

Analysis of regulatory elements involved in stress-induced and organ-specific expression of tobacco acidic and basic beta-1,3-glucanase genes

Plant Molecular Biology
M D Van de RheeJ F Bol

Abstract

Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethylene treatment and wounding strongly induce the basic glucanase genes but have little effect on genes encoding the acidic isoforms. Furthermore, the basic glucanase genes are constitutively expressed in roots and lower leaves of healthy plants, whereas the acidic glucanase genes are not. In order to investigate how these expression patterns are established, we fused promoter regions of an acidic and a basic glucanase gene to the beta-glucuronidase (GUS) reporter gene and examined expression of these constructs in transgenic tobacco plants. A fragment of 1750 bp and two 5'-truncated fragments of 650 bp and 300 bp of the acidic glucanase promoter were tested for induction of GUS gene expression after salicylate treatment and TMV infection. Upstream sequences of 1750 bp and 650 bp were sufficient for induction of the reporter gene by salicylate treatment and TMV infection, but the activity of the 300 bp fragment was s...Continue Reading

References

Jun 1, 1991·The Plant Cell·C P WoloshukB J Cornelissen
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C SperisenF Meins
Dec 1, 1990·Plant Molecular Biology·G PayneJ Ryals
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Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H J LinthorstJ F Bol
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Aug 1, 1969·Plant Physiology·S F Yang
Nov 1, 1987·Planta·G Felix, F Meins

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Citations

Dec 1, 1994·Plant Molecular Biology·D F Klessig, J Malamy
Apr 1, 1994·Plant Physiology·J. RyalsE. Ward
Jan 29, 2000·Nucleic Acids Research·S Kosugi, Y Ohashi
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·C. Gatz
Jul 19, 2008·Physiologia Plantarum·Antonio Jesús CastroChristophe Clément
Nov 1, 1993·Pharmazie in unserer Zeit·K Dombrowski, A W Alfermann
Nov 21, 2020·Current Genomics·Indrani BaruahGeetanjali Baruah

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