Analysis of reversions in the 5'-untranslated region of attenuated poliovirus after sequential administration of inactivated and oral poliovirus vaccines

The Journal of Infectious Diseases
Majid LaassriKonstantin Chumakov

Abstract

Replication of Sabin strains used in oral poliovirus vaccine (OPV) in the intestines of vaccine recipients leads to reversions that increase virus neurovirulence. Previously, a small study reported that prior immunization with inactivated poliovirus vaccine (IPV) resulted in faster accumulation of revertant virus, thus potentially increasing the risk of vaccine-associated paralytic poliomyelitis. We studied the impact that prior immunization with IPV and OPV has on shedding of revertant virus by healthy infants. By polymerase chain reaction (PCR), we amplified full-length poliovirus genomes directly from stool specimens from unimmunized infants and from infants previously immunized with IPV or OPV. The amplicons were used to quantify reversions in the 5'-untranslated region, using oligonucleotide microarray hybridization. Nearly all 140 samples that were PCR positive contained varying amounts of revertants of all 3 poliovirus serotypes. Polioviruses of Sabin types 2 and 3 reverted more easily than those of type 1. Prior vaccination with IPV did not increase the proportion of revertants after OPV administration.

Citations

Feb 12, 2008·Virus Genes·Christopher H LogueGregory J Atkins
Mar 10, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Bruce ThorleyHiroyuki Shimizu
Nov 22, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·María Silvina MunnéJorge E González
Mar 9, 2013·Risk Analysis : an Official Publication of the Society for Risk Analysis·Radboud J Duintjer TebbensKimberly M Thompson
Dec 1, 2011·The Journal of General Virology·Philip D Minor
Apr 26, 2007·Journal of Medical Virology·Majid LaassriKonstantin Chumakov
Dec 5, 2019·The Cochrane Database of Systematic Reviews·Agustín CiapponiAikant Bhatti
Mar 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Joshua D SnookJai S Rudra
Oct 31, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christopher van HoorebekeYvonne Maldonado

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