Analysis of rhamnolipid biosurfactants produced through submerged fermentation using orange fruit peelings as sole carbon source

Applied Biochemistry and Biotechnology
Seba George, K Jayachandran

Abstract

The fermentative production of rhamnolipid biosurfactant from Pseudomonas aeruginosa MTCC 2297 was carried out by submerged fermentation using various cost-effective waste materials such as orange peelings, carrot peel waste, lime peelings, coconut oil cake, and banana waste. The orange peel was found to be the best substrate generating 9.18 g/l of rhamnolipid biosurfactant with a surface tension reduction up to 31.3 mN/m. The production was growth independent, and optimum conditions were standardized. The emulsifying activity was highest against kerosene (73.3%). Rhamnolipid components were purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, high-performance liquid chromatography and thin-layer chromatography. The major rhamnolipid components were characterized, by fast atom bombardment mass spectrometry, as a mixture of dirhamnolipids and monorhamnolipids.

References

Jul 16, 1990·Biochimica Et Biophysica Acta·N B RendellP J Cole
Jan 24, 1998·Journal of Industrial Microbiology & Biotechnology·L SimZ Y Li
Nov 26, 1999·Applied Microbiology and Biotechnology·H S KimY Tani
Jun 16, 2000·Applied Microbiology and Biotechnology·I M BanatS S Cameotra
Oct 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S S WuA H Huang
Apr 17, 2002·Applied Microbiology and Biotechnology·R S Makkar, S S Cameotra
May 5, 2005·Applied and Environmental Microbiology·Nereus W GuntherDaniel K Y Solaiman

❮ Previous
Next ❯

Citations

Sep 13, 2011·AMB Express·Randhir S MakkarIbrahim M Banat
Jun 10, 2011·Journal of Basic Microbiology·Mital JadhavSanjay Govindwar
Apr 20, 2016·Bioresource Technology·Eduardo J GudiñaLígia R Rodrigues
Feb 15, 2018·Scientific Reports·Frederico Mendonça BahiaNádia Skorupa Parachin
Aug 21, 2013·Biotechnology Progress·Jenyffer Medeiros CamposIbrahim M Banat
Oct 3, 2019·World Journal of Microbiology & Biotechnology·Ángeles Domínguez RiveraVíctor Eric López Y López
May 5, 2016·Applied Biochemistry and Biotechnology·Rupshikha PatowarySuresh Deka
Aug 28, 2020·Applied Microbiology and Biotechnology·Jingjing JiangXuwei Long
May 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Abdul Hamid NurfarahinLai Yee Phang
Feb 6, 2017·Applied Biochemistry and Biotechnology·Mariaamalraj Samykannu, Anant Achary
Jun 10, 2018·Microbial Cell Factories·Yun Nian Tan, Qingxin Li
Dec 2, 2020·Bioresource Technology·Sunita VarjaniXuan-Thanh Bui
May 19, 2021·Analytical and Bioanalytical Chemistry·Maïssa DardouriIsabel A C Ribeiro
Jul 21, 2021·Environmental Science and Pollution Research International·Isabela Maria Monteiro VieiraDaniel Pereira Silva
Aug 10, 2021·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·A Landa-FazT G Roldán-Carillo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.