Abstract
Studies of the expression of selected genes within the intestinal mucosa will provide important new information about physiologic pathological processes that effect mucosal growth, differentiation, and function. To study gene expression in the gut, we developed a method to obtain sufficient undegraded RNA from human endoscopic intestinal biopsy specimens for Northern and slot blot analysis. To verify the method, we examined the differential expression of HLA class II genes in small intestinal mucosa. Levels of RNA transcripts for HLA-DR, -DP, and -DQ alpha and beta chains were assessed in freshly isolated endoscopic intestinal mucosal biopsy specimens and compared with levels in Epstein-Barr virus transformed B cells from the same individuals. Sufficient undegraded cellular RNA with distinct 28S and 18S ribosomal bands could be obtained from as few as two 2-3 mm endoscopic biopsies. Using chain and locus specific cDNA probes, HLA-DR, -DP, and -DQ subregion genes were shown to be expressed in intestinal mucosa, with the relative magnitude of RNA transcripts being DR greater than DP greater than DQ. The same hierarchy of expression was seen for EBV-transformed B cell lines. This method, in conjunction with the polymerase chain re...Continue Reading
References
Nov 27, 1979·Biochemistry·J M ChirgwinW J Rutter
Aug 1, 1986·Human Immunology·S F RadkaF M Brodsky
Apr 1, 1987·Analytical Biochemistry·P Chomczynski, N Sacchi
Feb 6, 1988·Lancet·J A NelsonJ A Levy
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M D HowellM F Kagnoff
Apr 8, 1988·Cell·D LoR L Brinster
Jul 1, 1986·The Journal of Experimental Medicine·M D HowellM F Kagnoff
Dec 1, 1986·Immunological Reviews·G F BottazzoR Pujol-Borrell
Feb 1, 1986·Human Immunology·P NataliS Ferrone
Nov 1, 1987·Scandinavian Journal of Immunology·H ScottE Thorsby
Jan 1, 1987·Journal of Pediatric Gastroenterology and Nutrition·J SarlesF Giraud
Dec 1, 1986·Digestive Diseases and Sciences·I HirataW O Dobbins
Jan 1, 1987·The Journal of Clinical Investigation·C A AlperE J Yunis
Jul 1, 1985·Immunological Reviews·A J KormanJ L Strominger
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 1, 1984·Biology of the Cell·J P GorvelC Mawas
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1983·Journal of Clinical Pathology·W S SelbyG Janossy
Sep 1, 1984·Transplantation·A S DaarP J Morris
Feb 1, 1984·Cell·B MalissenL Hood
Oct 1, 1983·Molecular and Cellular Biology·N J CowanD W Cleveland
Jan 1, 1980·Scandinavian Journal of Immunology·H ScottE Thorsby
Citations
May 1, 1990·Gut·M F Kagnoff
May 1, 1992·Gut·A J MacphersonT J Peters
Jul 23, 1998·Biology of Reproduction·J A ClapperG E Moss
Nov 26, 1998·Biology of Reproduction·L Y ChenY H Chen