Analysis of saccharides in beverages by HPLC with direct UV detection

Analytical and Bioanalytical Chemistry
Thomas SchmidWolfgang W Buchberger

Abstract

The present study demonstrates the suitability of direct UV detection for saccharide analysis in HPLC. Under highly alkaline conditions, the non-UV absorbing saccharides are converted by a photo-initiated chemical reaction in the detection cell into malonenolate, which can be detected at 266 nm. A straightforward method for such direct UV detection of saccharides after their separation by anion-exchange chromatography was developed and successfully applied to several beverage samples. Investigation and optimization of the influencing factors using design of experiment resulted in a baseline separation of glucose, fructose, and sucrose within 6 min and LOD values below 0.2 mg L(-1). In addition, a fast, simple and cost-effective flow injection method was developed to estimate the total saccharide concentration. The results of this method applied to beverage samples are in good agreement with the chromatographic method as well as to the saccharide concentration stated by the manufacturer. Finally, a comparison of different commercially available UV detectors and detector cells revealed that sensitive detection requires the use of recently introduced flow cells with extended path length.

References

Jul 31, 2007·Electrophoresis·Stella RovioHeli Sirén
Dec 15, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David J Harvey
Dec 28, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A I Ruiz-MatuteI Martínez-Castro
Oct 12, 2013·Electrophoresis·Virginia García-CañasAlejandro Cifuentes
Jun 5, 2015·Food Chemistry·A L GalantJ D Wilson

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Citations

Aug 12, 2017·Topics in Current Chemistry·Leslie BrownKenneth R Seddon
Aug 22, 2017·Analytical Methods : Advancing Methods and Applications·Gabe NagyNicola L B Pohl
Jun 27, 2021·Scientific Reports·Preeyarad CharoensumranThanit Praneenararat

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