Analysis of salivary peptides using HPLC-electrospray mass spectrometry

Biomedical Chromatography : BMC
Rui Miguel Pinheiro VitorinoFrancisco Manuel Lemos Amado

Abstract

Salivary peptides are involved in a wide range of functions constituting the first line of defence of oral cavity and precursors of dental pellicle formation. The presence of mucins in saliva makes difficult the analysis of the proteic content. This is due mainly to aggregation phenomenon between mucins and other high molecular weight glycoproteins and salivary proteins. Considering the importance of salivary peptides in biological functions, we have evaluated the influence of four different extraction methodologies on the separation and identification of these proteins by HPLC-MS. Based on their molecular weight, we identified a total of 22 peptides when extraction was performed using a solution of guanidine (6 m), compared with 14 peptides identified when saliva is acidified with TFA, which is an often used procedure. Our results also show the presence of mucin bind peptides, which include statherin, PRP1, PRP3, Histatin 1 and Histatin 5.

Citations

Jan 10, 2010·Food & Function·David Julian McClements, Yan Li
Jun 30, 2010·Molecular & Cellular Proteomics : MCP·Tiziana CabrasIrene Messana
Oct 25, 2008·Critical Reviews in Food Science and Nutrition·David Julian McClementsYeonhwa Park
Apr 3, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiuli SunEva J Helmerhorst
Oct 27, 2010·Expert Review of Proteomics·Francisco AmadoRui Vitorino
Aug 16, 2005·Expert Review of Proteomics·Francisco Manuel Lemos AmadoJosé Alberto Ramos Duarte
Feb 2, 2012·Expert Review of Proteomics·Massimo CastagnolaIrene Messana
May 12, 2012·Journal of Proteomics·Martine MorzelPatrick Ducoroy
Jan 23, 2009·Archives of Oral Biology·Melanie CampeseEva J Helmerhorst
Apr 15, 2009·Oral Diseases·T O R HjelmervikA I Bolstad
Apr 17, 2007·Annals of the New York Academy of Sciences·Eva J Helmerhorst
Jun 6, 2007·Biomedical Chromatography : BMC·T Cserháti
Aug 12, 2006·Archives of Oral Biology·E J HelmerhorstF G Oppenheim
Jul 20, 2012·Journal of Proteomics·Rui VitorinoFrancisco Amado
Jan 31, 2012·Archives of Oral Biology·Nuno RosaMarlene Barros
Feb 12, 2013·Archives of Oral Biology·Joel P ArraisJosé Luís Oliveira
Jul 27, 2007·Journal of Dental Research·E J Helmerhorst, F G Oppenheim
Oct 16, 2004·Biomedical Chromatography : BMC·Rui VitorinoFrancisco M L Amado
Nov 3, 2006·Proteomics·Rosanna InzitariMassimo Castagnola
May 23, 2007·Biomedical Chromatography : BMC·Rui VitorinoFrancisco M L Amado
Mar 8, 2005·Journal of Mass Spectrometry : JMS
Dec 1, 2007·Proteomics. Clinical Applications·Lucien F HarthoornEric Dransfield
Jul 14, 2009·Journal of Proteome Research·Tiziana CabrasIrene Messana

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.