Analysis of SARS-CoV-2 Antibodies in COVID-19 Convalescent Blood using a Coronavirus Antigen Microarray.

BioRxiv : the Preprint Server for Biology
Rafael Ramiro de AssisSaahir Khan

Abstract

The current practice for diagnosis of COVID-19, based on SARS-CoV-2 PCR testing of pharyngeal or respiratory specimens in a symptomatic patient at high epidemiologic risk, likely underestimates the true prevalence of infection. Serologic methods can more accurately estimate the disease burden by detecting infections missed by the limited testing performed to date. Here, we describe the validation of a coronavirus antigen microarray containing immunologically significant antigens from SARS-CoV-2, in addition to SARS-CoV, MERS-CoV, common human coronavirus strains, and other common respiratory viruses. A comparison of antibody profiles detected on the array from control sera collected prior to the SARS-CoV-2 pandemic versus convalescent blood specimens from virologically confirmed COVID-19 cases demonstrates near complete discrimination of these two groups, with improved performance from use of antigen combinations that include both spike protein and nucleoprotein. This array can be used as a diagnostic tool, as an epidemiologic tool to more accurately estimate the disease burden of COVID-19, and as a research tool to correlate antibody responses with clinical outcomes.

Citations

Jun 28, 2020·Journal of Virology·John P Moore, P J Klasse
Jul 18, 2020·Human Vaccines & Immunotherapeutics·Samuel A CohenOzlem Equils
Jul 11, 2020·The Cochrane Database of Systematic Reviews·Vanessa PiechottaNicole Skoetz
Oct 13, 2020·The Cochrane Database of Systematic Reviews·Khai Li ChaiNicole Skoetz
Jan 12, 2021·Journal of Proteome Research·Shalini AggarwalSanjeeva Srivastava
Feb 6, 2021·Scientific Reports·Tim A BrucknerBernadette Boden-Albala
Jan 13, 2021·Nature Medicine·Lisa E WagarMark M Davis
Mar 25, 2021·Scientific Reports·Christos FotisLeonidas G Alexopoulos
Apr 8, 2021·JMIR Public Health and Surveillance·Henry T PengAndrew Beckett
Dec 16, 2020·Journal of Clinical Microbiology·Dandan Li, Jinming Li
May 9, 2021·Journal for Immunotherapy of Cancer·Holden T Maecker
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May 25, 2021·Frontiers in Medicine·Yan MardianChuen-Yen Lau
Sep 8, 2021·Frontiers in Immunology·Luis A PeroniMarcio C Bajgelman

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Methods Mentioned

BETA
PCR
Protein Array

Software Mentioned

caret
combinat
R
R Foundation for Statistical
proprocessCore
pROC
ggplot2
stat
stats

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