PMID: 11311424Apr 20, 2001Paper

Analysis of sequences involved in IE2 transactivation of a baculovirus immediate-early gene promoter and identification of a new regulatory motif

Virus Research
C E Shippam-BrettD A Theilmann

Abstract

Opep-2 is a unique baculovirus early gene that has only been identified in the Orgyia pseudotsugata multiple capsid nucleopolyhedrovirus (OpMNPV). Previous analyses have shown this gene is expressed at very early times post-infection (p.i.) but is shut down by 36-48 h p.i. The promoter of opep-2 therefore, represents a class of early genes that is temporally regulated. In this study, a detailed analysis of the opep-2 promoter is performed to analyze the role individual motifs play in early gene expression. A new 13 base pair regulatory element was identified and shown to be essential in controlling high-level expression of this gene. In addition, mutational analysis revealed that GATA and CACGTG motifs, which have been shown to bind cellular factors in Sf9 and Ld652Y cells, played minor roles in influencing opep-2 expression in the absence of other viral factors. The OpMNPV transactivator IE2 causes a significant activation of the opep-2 promoter. Cotransfection of an extensive number of promoter deletions and mutations did not show any sequence specificity for IE2 transactivation. This is the first detailed analysis of the sequence requirements for IE2 transactivation, and these results suggest that IE2 does not bind directly ...Continue Reading

References

May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R R Hoopes, G F Rohrmann

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Citations

Mar 7, 2009·The Journal of General Virology·Jae Young ChoiYeon Ho Je
Feb 25, 2014·Insect Biochemistry and Molecular Biology·Liang Jiang, Qingyou Xia
Sep 28, 2004·The Journal of General Virology·Jeffrey M Slack, Martin Shapiro

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