Jun 9, 2020Paper

Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray

BioRxiv : the Preprint Server for Biology
Saahir KhanPrometheus Study Group

Abstract

The current practice for diagnosis of SARS-CoV-2 infection relies on PCR testing of nasopharyngeal or respiratory specimens in a symptomatic patient at high epidemiologic risk. This testing strategy likely underestimates the true prevalence of infection, creating the need for serologic methods to detect infections missed by the limited testing to date. Here, we describe the development of a coronavirus antigen microarray containing immunologically significant antigens from SARS-CoV-2, in addition to SARS-CoV, MERS-CoV, common human coronavirus strains, and other common respiratory viruses. A preliminary study of human sera collected prior to the SARS-CoV-2 pandemic demonstrates overall high IgG reactivity to common human coronaviruses and low IgG reactivity to epidemic coronaviruses including SARS-CoV-2, with some cross-reactivity of conserved antigenic domains including S2 domain of spike protein and nucleocapsid protein. This array can be used to answer outstanding questions regarding SARS-CoV-2 infection, including whether baseline serology for other coronaviruses impacts disease course, how the antibody response to infection develops over time, and what antigens would be optimal for vaccine development.

Citations

Jun 9, 2020·ELife·Michael J MinaBryan T Grenfell
Aug 4, 2020·Lab on a Chip·Per Niklas HeddeWeian Zhao
Aug 15, 2020·Nature Communications·Antonio E MuruatoPei-Yong Shi
May 14, 2020·Nature Medicine·Fatima AmanatFlorian Krammer
Sep 11, 2020·Nature Microbiology·Wen Shi LeeBrandon J DeKosky
Jun 26, 2020·The Cochrane Database of Systematic Reviews·Jonathan J DeeksCochrane COVID-19 Diagnostic Test Accuracy Group
Jul 24, 2020·International Journal of Molecular Sciences·Navid RabieeRajender S Varma
Jun 26, 2020·Frontiers in Immunology·Walter Fierz, Brigitte Walz
Sep 30, 2020·The Journal of Clinical Investigation·Massimiliano SecchiVito Lampasona
Nov 22, 2020·Analytical and Bioanalytical Chemistry·Mercy R BenzigarGuozhen Liu
Jan 8, 2021·The Analyst·Maria Ana Cristina Huergo, Nguyen Thi Kim Thanh
Jan 26, 2021·Archives of Virology·Sayeh DowlatshahiMohammad J Abdekhodaie
Feb 6, 2021·ACS Sensors·Guoqiang Liu, James F Rusling
Nov 13, 2020·Frontiers in Immunology·Alberto BerettaDonato Zipeto
Jan 16, 2021·Proceedings of the National Academy of Sciences of the United States of America·Varun SasisekharanUma Narayanasami
Mar 5, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Sophie EdouardMichel Drancourt
Mar 17, 2021·Journal of Clinical Immunology·Jennifer HicksKaitlyn Sadtler
Apr 3, 2021·International Journal of Molecular Sciences·Linhua TianLili Wang
Dec 16, 2020·Journal of Clinical Microbiology·Dandan Li, Jinming Li
Jun 3, 2021·Medical Sciences : Open Access Journal·Daniel KeaneyBrigid Lucey

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