Analysis of seroreactivity against cell culture-derived Bartonella spp. antigens in dogs

Journal of Veterinary Internal Medicine
B C HegartyEdward B Breitschwerdt

Abstract

Little is known about the specificity of Bartonella spp. immunofluorescent antibody (IFA) assays in dogs. Bacteremia in sick dogs most often has been associated with Bartonella henselae (Bh), Bartonella vinsonii subspecies berkhoffii (Bvb), and Bartonella koehlerae (Bk). Clarification of the diagnostic utility of IFA serology when testing against these organisms is needed. To evaluate the specificity of Bartonella IFA assays utilizing 6 cell culture-grown antigen preparations. Archived sera from SPF dogs (n = 29) and from dogs experimentally infected with Bvb (n = 10) and Bh (n = 3). Antibodies (Abs) to Bvb genotypes I, II, and III, Bh serotype I, strains H-1 and SA2, and to Bk were determined by IFA testing. Serum from naïve SPF dogs shown to be negative for Bartonella bacteremia did not react with any of the 6 Bartonella antigens by IFA testing. Dogs experimentally infected with Bvb genotype I developed Abs against homologous antigens, with no cross-reactivity to heterologous Bvb genotypes, Bh H-1, SA2 strains, or to Bk. Dogs experimentally infected with Bh serotype I developed Abs against Bh H-1, but not to Bh SA2 strain with no cross-reactive Abs to Bvb genotypes I-III or to Bk. Bartonella spp. Ab responses during acute exp...Continue Reading

References

Jan 1, 1995·Journal of Clinical Microbiology·E B BreitschwerdtK Wilson
Oct 15, 1996·Annals of Internal Medicine·D RaoultA M Sefton
Jun 11, 1998·Emerging Infectious Diseases·D L Kordick, E B Breitschwerdt
Jul 13, 2000·Veterinary Immunology and Immunopathology·B L PappalardoE B Breitschwerdt
Aug 24, 2001·Journal of the American Veterinary Medical Association·T E HonadelT B Farver
Dec 4, 2001·Veterinary Immunology and Immunopathology·B L PappalardoE B Breitschwerdt
May 31, 2002·Journal of Clinical Microbiology·Bernard La ScolaDidier Raoult
Aug 16, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Edward B BreitschwerdtBarbara C Hegarty
Sep 17, 2004·Veterinary Research·Laia Solano-GallegoEdward Breitschwerdt
Mar 28, 2008·Journal of Clinical Microbiology·Maria B CadenasEdward B Breitschwerdt
Jun 23, 2009·Annals of the New York Academy of Sciences·Bruno B ChomelE B Breitschwerdt
Aug 28, 2009·Journal of Veterinary Internal Medicine·M VaranatE B Breitschwerdt
Mar 17, 2010·Journal of Veterinary Emergency and Critical Care·Edward B BreitschwerdtMichael R Lappin
Jun 24, 2010·Veterinary Microbiology·Ying BaiLeonard F Peruski
Sep 14, 2010·International Journal of Medical Microbiology : IJMM·Patrick O KaiserVolkhard A J Kempf
May 28, 2011·Journal of Veterinary Internal Medicine·C PérezE B Breitschwerdt
Jul 9, 2011·Vector Borne and Zoonotic Diseases·Natalie A CherryEdward B Breitschwerdt
Mar 28, 2012·Medical Microbiology and Immunology·Christiane BeerlageEdward B Breitschwerdt
Apr 21, 2012·Emerging Infectious Diseases·Ricardo G MaggiEdward B Breitschwerdt
Oct 3, 2012·PLoS Neglected Tropical Diseases·Bruno B ChomelEdward B Breitschwerdt

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Citations

Mar 29, 2014·Parasites & Vectors·Ricardo G MaggiEdward B Breitschwerdt
Oct 18, 2014·Vector Borne and Zoonotic Diseases·Julie M BradleyEdward B Breitschwerdt
Nov 13, 2015·Veterinary Ophthalmology·Maggie R JinksBrian C Gilger
Mar 28, 2017·Vector Borne and Zoonotic Diseases·Josipa KulešMangesh Bhide
Mar 15, 2018·Revista Do Instituto De Medicina Tropical De São Paulo·Beatriz Teixeira Gomes da SilvaNádia Regina Pereira Almosny
Mar 6, 2015·Journal of Clinical Microbiology·Ricardo G MaggiEdward B Breitschwerdt
Dec 28, 2018·Vector Borne and Zoonotic Diseases·Edward B BreitschwerdtJulie M Bradley
Jan 1, 2020·Journal of Veterinary Internal Medicine·Erin LashnitsEdward B Breitschwerdt
Mar 8, 2020·Pathogens·Aránzazu PortilloEdward Breitschwerdt
Sep 27, 2018·Medical Microbiology and Immunology·Edward B Breitschwerdt, Ricardo G Maggi
Dec 6, 2018·Parasites & Vectors·Alejandra Álvarez-FernándezLaia Solano-Gallego
Jan 17, 2020·Journal of Clinical Microbiology·Pradeep NeupaneEdward B Breitschwerdt
Jan 31, 2017·Veterinary Dermatology·Edward B Breitschwerdt
May 28, 2019·Journal of Veterinary Internal Medicine·Erin LashnitsEdward B Breitschwerdt
Dec 3, 2017·Journal of Veterinary Internal Medicine·E LashnitsE B Breitschwerdt
Jan 10, 2020·Journal of Veterinary Internal Medicine·Emily K PurswellShelly L Vaden
Nov 18, 2021·Journal of Veterinary Internal Medicine·Erin LashnitsEdward B Breitschwerdt

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Methods Mentioned

BETA
ELISA
PCR

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