Analysis of short tandem repeats by parallel DNA threading.

PloS One
Pawel ZajacAfshin Ahmadian

Abstract

The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the number of targeted STRs.

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May 14, 2011·Molecular Ecology Resources·E GuichouxR J Petit
May 28, 2010·Insect Molecular Biology·H Charles J Godfray
Dec 3, 2014·Genomics, Proteomics & Bioinformatics·Yaran YangJiangwei Yan
Apr 30, 2013·Scientific Reports·Vipul BhargavaShankar Subramaniam
Jun 1, 2021·Journal of Molecular Neuroscience : MN·Jalal GharesouranMaryam Rezazadeh

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Methods Mentioned

BETA
PCR
electrophoresis
genotyping

Software Mentioned

GeneScan

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