PMID: 6984382Jan 1, 1982Paper

Analysis of single and twin sister chromatid exchanges in endoreduplicated normal and Bloom syndrome B-lymphoid cells

Chromosoma
Y ShiraishiA A Sandberg

Abstract

Single and twin sister chromatid exchanges (SCEs) were analysed in the colcemid-induced endoreduplicated normal and Bloom syndrome (BS) B-lymphoid cells with diplochromosomes. In normal cells, an equal number of SCEs occur in each of the two cell cycles; the ratio of single (= 5.51 SCEs/cell) to twins (= 2.64 SCEs/cell) was 2:1 on the endoreduplicated-cell basis, and it was 1:1 on the diploid-cell basis. In contrast, in 29 endomitoses from one BS B-lymphoid line, a manyfold increase of single SCEs was detected and 139.4 single SCEs on the average were counted, whereas twin SCEs were rare and only 4.9 twin SCEs were countable. In BS cells, the ratio of single (= 139.4 SCEs/cell) to twins (4.9 SCEs/cell) was 28:1 on the endoreduplicated-cell basis, and it was 14:1 on the diploid cell-basis; the rates of S1 and S2 exchanges were 4.9 and 69.7 SCEs/cell, respectively. The present study strongly indicates that most of BS SCEs occur during the second cell cycle when BrdU-containing DNA is used as template for replication and that BrdU enhances BS SCEs.

References

Feb 15, 1976·International Journal of Cancer. Journal International Du Cancer·N Yamamoto, Y Hinuma
Jan 1, 1976·Cytogenetics and Cell Genetics·Y ShiraishiA A Sandberg
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R S ChagantiJ German
Dec 31, 1980·Biochemical and Biophysical Research Communications·A OikawaT Sugimura

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Citations

Dec 1, 1986·Human Genetics·H Joenje, A B Oostra
Dec 1, 1995·Genome Génome / Conseil National De Recherches Canada·A ZambetakiP Mavragani-Tsipidou
Jan 31, 2014·Radiation and Environmental Biophysics·F Andrew RayMichael N Cornforth
Oct 1, 1988·Human Genetics·T LukusaJ J Cassiman
Jan 1, 1985·Critical Reviews in Clinical Laboratory Sciences·A A Sandberg
May 31, 1983·Biochemical and Biophysical Research Communications·S BanT Sugahara
May 18, 2016·Nucleic Acids Research·Niek van Wietmarschen, Peter M Lansdorp
Nov 10, 2001·Annual Review of Genomics and Human Genetics·A J van BrabantN A Ellis
Jan 1, 1989·Journal of Toxicology and Environmental Health·B A SchaumannM Pederson
May 1, 1987·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·M J MehtaR G Bhatt

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