Analysis of single nucleic acid molecules with protein nanopores.

Methods in Enzymology
Giovanni MagliaHagan Bayley

Abstract

We describe the methods used in our laboratory for the analysis of single nucleic acid molecules with protein nanopores. The technical section is preceded by a review of the variety of experiments that can be done with protein nanopores. The end goal of much of this work is single-molecule DNA sequencing, although sequencing is not discussed explicitly here. The technical section covers the equipment required for nucleic acid analysis, the preparation and storage of the necessary materials, and aspects of signal processing and data analysis.

Citations

Apr 18, 2013·Nature Communications·Shiksha MantriHagan Bayley
Sep 13, 2013·Nature Communications·Lorenzo FranceschiniGiovanni Maglia
Nov 8, 2011·Nature Nanotechnology·Gabriel VillarHagan Bayley
Dec 4, 2012·Nucleic Acids Research·Dylan ChivianAdam P Arkin
Apr 17, 2016·The Journal of Chemical Physics·Debasish Mondal, M Muthukumar
Jan 1, 2012·Worm·Sujai KumarMark Blaxter
Sep 3, 2016·The Journal of Chemical Physics·Debasish Mondal, M Muthukumar
May 23, 2017·ACS Nano·Garbiñe CelayaDavid Rodriguez-Larrea
Sep 10, 2014·Nature Communications·David Rodriguez-Larrea, Hagan Bayley
Mar 12, 2013·Nature Nanotechnology·David Rodriguez-Larrea, Hagan Bayley
Jan 30, 2016·Science Advances·Ching-Wen HoGiovanni Maglia
Sep 26, 2013·Biochemical Society Transactions·Shiksha Mantri, K Tanuj Sapra
Apr 7, 2020·Nature Chemistry·Nicole Stéphanie GalenkampGiovanni Maglia
Oct 4, 2019·Scientific Reports·Madiha Sultan, Anastassia Kanavarioti
May 14, 2020·The Journal of Biological Chemistry·Katarina Bartoš JansenColin Kleanthous
May 22, 2018·Chemical Science·Jéssica RodríguezJosé L Mascareñas
Apr 5, 2020·Communications Biology·Jianfei FengDavid Rodriguez-Larrea
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May 12, 2021·Nature Chemistry·Alistair J ScottDerek N Woolfson
May 25, 2021·Frontiers in Chemistry·Hongcheng YangRui Hao
Dec 22, 2020·Nano Letters·Mariam BayoumiGiovanni Maglia
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