Analysis of Sir2E in the cellular slime mold Dictyostelium discoideum: cellular localization, spatial expression and overexpression

Development, Growth & Differentiation
Takahiro Katayama, Hiro Yasukawa

Abstract

It has been reported that Dictyostelium discoideum encodes four silent information regulator 2 (Sir2) proteins (Sir2A-D) showing sequence similarity to human homologues of Sir2 (SIRT1-3). Further screening in a database revealed that D. discoideum encodes an additional Sir2 homologue (Sir2E). The amino acid sequence of Sir2E is not similar to those of SIRTs but is similar to those of proteins encoded by Giardia lamblia, Cryptosporidium hominis and Cryptosporidium parvum. Fluorescence of Sir2E-green fluorescent protein fusion protein was detected in the D. discoideum nucleus, indicating that Sir2E is a nuclear localizing protein. Reverse transcription-polymerase chain reaction and whole-mount in situ hybridization analyses showed that D. discoideum expressed sir2E in amoebae in the growth phase and in prestalk cells in the developmental phase. D. discoideum overexpressing sir2E grew faster than the wild type. These results indicate that Sir2E plays important roles both in the growth phase and developmental phase of D. discoideum.

References

Sep 1, 1970·The Biochemical Journal·D J Watts, J M Ashworth
Sep 1, 1970·The Biochemical Journal·J M Ashworth, D J Watts
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Oct 1, 1996·Current Opinion in Genetics & Development·R A Firtel
Jun 30, 2000·Biochemical and Biophysical Research Communications·R A Frye
Jul 2, 2002·The Journal of Biological Chemistry·Jeong-Ho ChangYunje Cho
Jan 27, 2004·Journal of Leukocyte Biology·Stephen W BuckJeffrey S Smith
May 28, 2004·Molecular Biology and Evolution·Le Sy Vinh, Arndt Von Haeseler
Jun 11, 2004·Annual Review of Biochemistry·Gil Blander, Leonard Guarente
Aug 5, 2005·Molecular Biology of the Cell·Eriko MichishitaIzumi Horikawa
Aug 27, 2005·Current Opinion in Chemical Biology·John M Denu
Jan 2, 2007·The Journal of Biological Chemistry·Masaya TannoYoshiyuki Horio
Jan 1, 2008·Microbes and Environments·Takahiro Katayama, Hiro Yasukawa

❮ Previous
Next ❯

Citations

Jun 22, 2010·Parasitology Research·Hiro Yasukawa, Kenji Yagita
Aug 24, 2013·PloS One·Edward Roshan MirandaGad Shaulsky
Dec 29, 2010·Biochemical and Biophysical Research Communications·Annette Müller-TaubenbergerSandra B Hake

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.