Analysis of small carbohydrates in several bioactive botanicals by gas chromatography with mass spectrometry and liquid chromatography with tandem mass spectrometry

Journal of Separation Science
Serban C MoldoveanuJeff Zhu

Abstract

Bioactive botanicals contain natural compounds with specific biological activity, such as antibacterial, antioxidant, immune stimulating, and taste improving. A full characterization of the chemical composition of these botanicals is frequently necessary. A study of small carbohydrates from the plant materials of 18 bioactive botanicals is further described. The study presents the identification of the carbohydrate using a gas chromatographic-mass spectrometric analysis that allows detection of molecules as large as maltotetraose, after changing them into trimethylsilyl derivatives. A number of carbohydrates in the plant (fructose, glucose, mannose, sucrose, maltose, xylose, sorbitol, and myo-, chiro-, and scyllo-inositols) were quantitated using a novel liquid chromatography with tandem mass spectrometric technique. Both techniques involved new method developments. The gas chromatography with mass spectrometric analysis involved derivatization and separation on a Rxi(®)-5Sil MS column with H2 as a carrier gas. The liquid chromatographic separation was obtained using a hydrophilic interaction type column, YMC-PAC Polyamine II. The tandem mass spectrometer used an electrospray ionization source in multiple reaction monitoring po...Continue Reading

References

Sep 27, 2005·Journal of the American Society for Mass Spectrometry·Eduard RogatskyDaniel Stein
May 23, 2006·Journal of Chromatography. a·Rune Slimestad, Ingunn M Vågen
Apr 5, 2011·Analytical and Bioanalytical Chemistry·G McRae, C M Monreal
Dec 19, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhiqian Liu, Simone Rochfort
Jan 10, 2013·Scientific Reports·Sajia AkhterRobert A Edwards
Jan 27, 2015·Journal of Chromatography. a·Serban Moldoveanu, Victor David

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