Analysis of SNPs and other genomic variations using gel-based chips

Human Mutation
Alexander Kolchinsky, A D Mirzabekov

Abstract

Application of microarrays for the analysis of point mutations and SNPs in genomic DNAs is currently under intensive development. Various technologies are being investigated, employing enzymatic, chemical, and physical tools [for review, see Tillib and Mirzabekov, 2001]. Our current approach is based on the use of IMAGE chips (immobilized microarrays of gel elements) consisting of an array of gel pads attached to a hydrophobic glass surface. The gel pads range in size from picoliters to nanoliters and are used for immobilization of oligonucleotide probes, as well as miniature test tubes for chemical or enzymatic reactions with tethered compounds. Nucleic acids are hybridized, fractionated, modified, and subjected to enzymatic reactions inside the pads. All steps of sequence analysis (PCR-amplification, activation or release of primers and products, DNA extension, hybridization, and reading of the results) can be performed within the same pad. A flexible and inexpensive technology platform enables one to monitor processes in the arrays in both real time and steady-state. Identification of SNPs, microsequencing, and other specific tasks are easily performed. In particular, stacking interactions with short oligonucleotides enhance...Continue Reading

References

Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·K R KhrapkoA D Mirzabekov
Oct 9, 1989·FEBS Letters·K R KhrapkoA D Mirzabekov
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·G YershovA Mirzabekov
Oct 1, 1995·Clinical Microbiology Reviews·J M Musser
Aug 15, 1996·Nucleic Acids Research·E TimofeevV L Florentiev
Nov 15, 1996·Nucleic Acids Research·D Proudnikov, A Mirzabekov
Mar 15, 1997·Nucleic Acids Research·M S ShchepinovE M Southern
Jun 1, 1997·Applied and Environmental Microbiology·D Y GuschinA D Mirzabekov
Apr 29, 1998·Nucleic Acids Research·A V FotinA D Mirzabekov
Dec 24, 1998·Nucleic Acids Research·F N RehmanT C Boles
Sep 18, 1999·BioTechniques·A V VasiliskovA D Mirzabekov
Feb 8, 2000·Analytical Biochemistry·P ArenkovA Mirzabekov
Jul 8, 2000·Biologicals : Journal of the International Association of Biological Standardization·D ProudnikovA Mirzabekov
Oct 6, 2000·Journal of Biomolecular Structure & Dynamics·V R ChechetkinA D Mirzabekov
Feb 7, 2001·Applied and Environmental Microbiology·S G BavykinA D Mirzabekov
Feb 13, 2001·Current Opinion in Biotechnology·S V Tillib, A D Mirzabekov

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Citations

Apr 1, 2003·Mass Spectrometry Reviews·Jörg Tost, Ivo G Gut
Jul 6, 2006·Analytical and Bioanalytical Chemistry·Johnson Kian-Kok Ng, Wen-Tso Liu
May 15, 2012·Analytical and Bioanalytical Chemistry·K TsougeniE Gogolides
Oct 5, 2007·Journal of Materials Science. Materials in Medicine·Seiji YamaguchiTakeshi Yao
Aug 5, 2003·Analytical Biochemistry·Robi D MitraGeorge M Church
Nov 18, 2003·Journal of Biomolecular Structure & Dynamics·Dmitrii V PyshnyiEugenia M Ivanova
May 19, 2007·Journal of Biomolecular Structure & Dynamics·N V SorokinA S Zasedatelev
Jul 9, 2009·Journal of Biomolecular Structure & Dynamics·S V Pan'kovA V Chudinov
Apr 16, 2005·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Jacquelyn J Bower, Xianglin Shi
May 12, 2009·Molekuliarnaia biologiia·V E Ramenskiĭ, Sh R Siuniaev
Feb 11, 2010·Future Microbiology·Tomasz A LeskiBaochuan Lin
Nov 5, 2008·Biosensors & Bioelectronics·Jing Tang, Pengfeng Xiao
Mar 1, 2005·Trends in Biotechnology·Rastislav Levicky, Adrian Horgan
Sep 27, 2005·Journal of Biotechnology·D A ZubtsovA S Zasedatelev
Aug 8, 2008·Biosensors & Bioelectronics·B Ramana MurthyW T Liu
Mar 30, 2004·Biopolymers·Arnold Vainrub, B Montgomery Pettitt
May 1, 2007·Influenza and Other Respiratory Viruses·Eugeny E FesenkoAlexander S Zasedatelev
Mar 14, 2008·The Journal of Physical Chemistry. B·Bat Ami GotlivRon Naaman
Dec 8, 2011·Analytical Chemistry·Avraham BrombergRichard A Mathies
Apr 16, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Martin RendlJürgen Rühe

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