PMID: 8960127Dec 2, 1996Paper

Analysis of spontaneous and double-strand break-induced recombination in rad mutants of S. pombe

Mutation Research
E A FortunatoS Subramani

Abstract

Schizosaccharomyces pombe strains containing direct repeats of adeó heteroalleles separated by a functional uro4+ gene, and a DNA site for induction of a double-strand break (DSB), have been used to analyze pathways of spontaneous and DSB-induced intrachromosomal mitotic recombination. These substrates yield Ade+ Ura+ convertants or Ade+ Ura- deletions, by the DSB/gap repair and single-strand annealing (SSA) pathways of recombination, respectively. In S. cerevisiae, the DSB/gap repair pathway is RAD52 dependent, and the RAD1 and RAD10 genes are involved in the SSA pathway. We have sought to understand the genetic control of the pathways of mitotic recombination in S. pombe by determining the effects of mutations in six rad genes involved in DNA repair: rad1 and rad3 involved in checkpoint control in response to unreplicated or damaged DNA; rad5 (homologue of S. cerevisiae RAD3) and rad10 (homologue of S. cerevisiae RAD1) involved in nucleotide excision repair; rad21 and rad22 (homologue of S. cerevisiae RAD52) involved in the repair of ionizing radiation-induced DNA damage. The results suggest that the genetic control of the pathways of spontaneous and DSB-induced mitotic intrachromosomal recombination in S. pombe is different ...Continue Reading

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Citations

May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·D van HeemstD Zickler
Aug 28, 2004·Molecular Biology of the Cell·Stephanie PebernardMichael N Boddy
Jun 17, 2009·Molecular and Cellular Biology·Emily A OutwinMatthew J O'Connell
Jul 2, 2014·Chromosoma·Claudia Tapia-AlvealMatthew J O'Connell
Mar 21, 1998·Radiation Oncology Investigations·C W StevensC J Koch
Jan 16, 2020·Genes·Hannah M HyltonRuben C Petreaca
Aug 25, 2000·The Journal of Biological Chemistry·W J KimS D Park
Aug 15, 2003·Molecular and Cellular Biology·Li-Lin DuPaul Russell

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