Analysis of Sporothrix schenckii sensu stricto and Sporothrix brasiliensis virulence in Galleria mellonella

Journal of Microbiological Methods
Diana M Clavijo-GiraldoHéctor M Mora-Montes

Abstract

The study of the host-pathogen interaction is essential to understand the mechanisms underlying adhesion, colonization and tissue damage by pathogens. This is usually achieved by performing in vivo studies using small mammals, such as rats, mice and guinea pigs. Nowadays, the mouse models of systemic or subcutaneous infection are the gold standard assays to analyze the virulence of members of the Sporothrix schenckii complex. There are, however, invertebrates that have been recently used as alternative hosts to assess the virulence of both bacteria and fungi, and among them, larvae of Galleria mellonella are popular because they are easy to breed, and require non-specialized facilities to maintain the colony. Here, we assessed the use of G. mellonella larvae to test the virulence of S. schenckii sensu stricto and Sporothrix brasiliensis strains, and found that infection with yeast-like cells, but not with conidia or germlings, reproduces the virulence data generated in the mouse model of infection. Furthermore, with this insect model we could classify the virulence of some strains as low, intermediate or high, in line with the observations in the mammalian model. Therefore, G. mellonella is suitable, and a new alternative, to t...Continue Reading

References

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Oct 11, 2015·Journal of Microbiological Methods·Patrícia Canteri de SouzaRicardo Sergio Almeida

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Citations

Feb 9, 2017·Frontiers in Microbiology·Liliana ScorzoniAna M Fusco-Almeida
Mar 12, 2019·Future Microbiology·José A Martínez-ÁlvarezHéctor M Mora-Montes
Aug 8, 2018·Medical Mycology·Luana RossatoSandro Rogério de Almeida
Mar 4, 2020·Journal of Fungi·Camila BonicheCarlos Pelleschi Taborda
Aug 31, 2017·PLoS Neglected Tropical Diseases·Paula Portella Della TerraZoilo Pires de Camargo
Oct 24, 2018·Journal of Fungi·Junya L SingulaniMaria José S Mendes-Giannini
Sep 2, 2020·Journal of Fungi·Laura C García-CarneroHéctor M Mora-Montes
Feb 9, 2021·Frontiers in Cellular and Infection Microbiology·Nathália Ferreira FregoneziAna Marisa Fusco-Almeida
Jun 12, 2021·Infection and Drug Resistance·Nancy E Lozoya-PérezHéctor M Mora-Montes
Jul 27, 2021·The Cell Surface·Alma K Tamez-CastrellónHéctor M Mora-Montes
Jun 22, 2021·Antimicrobial Agents and Chemotherapy·Luana Pereira Borba-SantosSonia Rozental

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