Analysis of structure and gene expression of bovine CCDC3 gene indicates a function in fat metabolism

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Annett EberleinRosemarie Weikard

Abstract

Our study reports the molecular analysis of the bovine gene encoding the coiled-coil domain-containing protein 3 (CCDC3). Based on comparative sequence analysis and in silico sequence merging of predicted gene models, a new full-length gene model for the bovine CCDC3 gene was predicted and confirmed experimentally. The CCDC3 gene was assigned to bovine chromosome 13. It consists of three exons comprising 2599bp encoding for a respective protein of 274 amino acids. The strong CCDC3 sequence homology on amino acid level between species suggests a conserved universal function of this protein. In mice, the CCDC3 gene had been found to be highly expressed in adipocytes and regulated by hormonal-nutritional alternations and in obesity. The tissue expression pattern of bovine CCDC3 mRNA indicates a ubiquitous physiological function of the gene. Significant differences in CCDC3 mRNA expression in skeletal muscle between individuals characterized by divergent intramuscular fat deposition support the potential function of the gene in fat or energy metabolism, which possibly could also be inferred for other mammalian species. This first report of structural analysis and molecular characterization of the CCDC3 gene in cattle will contribut...Continue Reading

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Citations

Oct 28, 2016·Molecular Ecology·Makoto KusakabeJun Kitano
Mar 7, 2014·Indian Journal of Orthopaedics·Kp SrikanthS Rajasekaran
Jan 22, 2015·The Journal of Biological Chemistry·Sachiko KobayashiIichiro Shimomura
Aug 23, 2017·Scientific Reports·Wenjuan LiaoHua Lu
May 5, 2021·Reproductive Sciences·Patra Priyadarshini Priyanka, Suresh Yenugu

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