Analysis of sub μg/kg lincomycin in honey, muscle, milk, and eggs using fast liquid chromatography-tandem mass spectrometry

Journal of Chromatographic Science
Adám TölgyesiEdina Tóth

Abstract

A fast liquid chromatography-tandem mass spectrometry (LC-MS-MS) method is developed to determine lincomycin (LM) in honey, muscle, milk, and egg. Samples are cleaned-up at pH 4.7 using Strata-X-C mixed-mode polymeric strong cation exchange solid-phase extraction (SPE) cartridges, which could selectively adsorb the lincomycin from matrices under the acidic condition. LM is separated on the recently introduced Kinetex XB core-shell type HPLC column using isocratic elution mode with a mobile phase containing 0.1% formic acid in water/acetonitrile (93/7, v/v, pH 2.6) at a flow rate of 0.7 mL/min. The subsequent MS/MS detection has decreased ion effect, which allows the limit of detection (LOD) of LM for honey to be 0.05 µg/kg for honey and 0.5 µg/kg for muscle, milk, and egg. These LODs are much lower than those reported previously. The other main advantage of the developed method is the analysis time of only 3.5 min, which is about three times shorter than other reported LC-MS-MS methods. Recoveries varies between 94.2% and 125.2% and in-house reproducibility ranges from 3.7% to 28.7%. The developed method is validated according to European Union (EU) Commission Decision 2002/657/EC using a matrix-comprehensive validation strateg...Continue Reading

References

May 7, 2004·Journal of Pharmaceutical and Biomedical Analysis·Della Wai-mei SinAlec Chun-bong Ip
Sep 27, 2005·Journal of Pharmaceutical and Biomedical Analysis·Michal DousaKarel Lemr
Feb 9, 2008·Journal of Agricultural and Food Chemistry·Mayda I LopezPak-Sin Chu
Feb 2, 2010·Journal of Chromatography. a·Fabrice GrittiGeorges Guiochon
May 12, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Adám TölgyesiJeno Fekete
Aug 20, 2010·Journal of Agricultural and Food Chemistry·Maksim A Burkin, Inna A Galvidis
Oct 14, 2010·Journal of Pharmaceutical and Biomedical Analysis·Szabolcs FeketeJeno Fekete
Jan 19, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Adám TölgyesiJeno Fekete
Apr 19, 2011·Drug Testing and Analysis·Victoria F Samanidou, Eftichia G Karageorgou

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