PMID: 8594538Oct 1, 1995Paper

Analysis of synaptic transmission at single identified boutons on rat spinal neurons in culture

Pflügers Archiv : European journal of physiology
K VogtJ Streit

Abstract

The spatial organization of receptor channels has a major influence on the speed and possible plasticity of synaptic signal transmission. We have studies glutamatergic synapses on neurons in organotypic cultures of rat spinal cord. In order to avoid the problems related to the analysis of currents of unknown origin within a neuron, we chose to examine the functional properties of single identified synapses. Iontophoretic mapping of the cell surface revealed hot spots of high glutamate sensitivity coincident with presynaptic boutons stained with the dye FM 1-43. Local application of KCl to these sites caused bursts of synaptic release. Hot spots typically consisted of 330 receptors with an average single-channel conductance of 8.3 pS. Evoked synaptic currents involved only about 40-50 receptors and nevertheless showed characteristics of saturation. This suggests that glutamate receptor clusters at sites of presynaptic terminals are organized into well separated subclusters opposite release sites.

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Citations

Aug 2, 2002·European Journal of Pharmacology·S A Fedulova, N S Veselovsky
Mar 12, 2002·The European Journal of Neuroscience·Pascal DarbonJürg Streit
May 1, 1997·The European Journal of Neuroscience·K VogtH R Lüscher
Apr 15, 1999·Annual Review of Neuroscience·A J CochillaW J Betz
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·A K McAllister, C F Stevens
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·S KirischukR Grantyn
Jan 13, 2004·Physiological Reviews·Matthew A Xu-Friedman, Wade G Regehr
May 10, 2005·Journal of Neuroscience Research·Uwe-Robert KochRüdiger Köhling
Oct 6, 1997·Journal of Neurophysiology·M FrerkingM Wilson
May 20, 1998·Journal of Neurophysiology·B X GaoL Ziskind-Conhaim

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