PMID: 9444944Jan 28, 1998Paper

Analysis of synergism between stem cell factor and granulocyte-macrophage colony-stimulating factor on human megakaryoblastic cells: an increase in tyrosine phosphorylation of 145 kDa subunit of c-kit in two-factor combination

Leukemia Research
T KamijoAtsushi Komiyama

Abstract

In normal hematopoiesis, stem cell factor (SCF) stimulates survival, proliferation and differentiation of hematopoietic progenitors. Although SCF acts synergistically with a variety of cytokines, the mechanism of growth factor-cooperation remains to be determined. To analyze the synergism between SCF and granulocyte-macrophage colony-stimulating factor (GM-CSF), we established a new megakaryoblastic cell line, HML-2, by culture in the presence of both SCF and GM-CSF. While SCF alone or GM-CSF alone supported modest cell growth, SCF and GM-CSF together induced substantial growth of this cell line. SCF alone tyrosine-phosphorylated several bands including the 145 kDa subunit of c-kit. GM-CSF alone did not cause the tyrosine phosphorylation of the 145 kDa subunit, but markedly up-regulated the expression of the 145 kDa subunit of c-kit. The combination of SCF and GM-CSF resulted in a synergistic increase in tyrosine phosphorylation of the 145 kDa subunit of c-kit. Several proliferation inhibitors which removed the two-factor interaction on the growth of the HML-2 cells down-regulated the 145 kDa subunit of c-kit. Thus, a synergistic increase in tyrosine phosphorylation of the 145 kDa subunit of c-kit may be one possible mechanism ...Continue Reading

References

Aug 1, 1992·Journal of Biochemistry·M ImotoK Umezawa
May 1, 1991·Molecular and Cellular Biology·M J Welham, J W Schrader
Jul 1, 1991·The Journal of Experimental Medicine·M OgawaS Nishikawa
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Nov 1, 1994·Experimental Cell Research·M ImotoK Umezawa

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Citations

Sep 10, 2003·Journal of Leukocyte Biology·Kouichi TakeuchiAtsushi Komiyama
Dec 31, 2004·Stem Cells·Johan LennartssonR Shivakrupa
Oct 18, 2012·Physiological Reviews·Johan Lennartsson, Lars Rönnstrand
Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·D Linnekin

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