PMID: 9650719Jul 3, 1998Paper

Analysis of synthetic peptides by capillary zone electrophoresis in organic/aqueous buffers

The Journal of Peptide Research : Official Journal of the American Peptide Society
C Miller, J Rivier

Abstract

Whereas synthetic peptides have been routinely analyzed for purity by reverse phase high performance liquid chromatography (RPHPLC) for a number of years, it is only in the last decade that the use of capillary zone electrophoresis (CZE) in aqueous buffers has been taken advantage of as an orthogonal method for the detection of impurities. However, we have found that hydrophobic amino acids and peptides often migrate as very broad, tailing absorbances or even precipitate in the aqueous buffers during CZE analysis. As a result, alternative buffer systems containing organic modifiers were sought. Varying concentrations of acetonitrile, methanol and isopropanol in sodium phosphate and triethylammonium phosphate buffers were used to study their effects on the electrophoretic migration of several synthetic peptides [gonadotropin releasing hormone (GnRH), corticotropin releasing factor (CRF) and analogs] and an enantiomeric synthetic amino acid. The organic/aqueous buffers used to obtain the best conditions for separation of porcine gonadotropin-releasing hormone (GnRH) and chicken II GnRH were then used to optimize a separation of nine native forms of GnRH decapeptides. Interestingly, several of these GnRHs have identical formal cha...Continue Reading

References

Dec 1, 1990·Journal of Neurobiology·M R Davis, R D Fernald
Sep 23, 1983·Journal of Chromatography·J Rivier, R McClintock
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F PowellN M Sherwood
Apr 1, 1993·Endocrine Reviews·N M SherwoodI R Coe
Oct 1, 1993·Journal of Medicinal Chemistry·J F HernandezJ Rivier

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Citations

Dec 22, 1999·Electrophoresis·V Kasicka
Oct 26, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·D BonnetO Melnyk
Apr 16, 2008·Journal of Medicinal Chemistry·Christy Rani R GraceJean E Rivier
Apr 16, 2008·Journal of Medicinal Chemistry·Judit ErchegyiJean E Rivier
Apr 9, 2009·Journal of Medicinal Chemistry·Judit ErchegyiJean Claude Reubi
Jul 13, 2004·Toxicon : Official Journal of the International Society on Toxinology·Russell W TeichertBaldomero M Olivera

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