PMID: 11337383May 5, 2001Paper

Analysis of T-cell subpopulations in T-cell non-Hodgkin's lymphoma of angioimmunoblastic lymphadenopathy with dysproteinemia type by single target gene amplification of T cell receptor- beta gene rearrangements

The American Journal of Pathology
K WillenbrockM L Hansmann

Abstract

Angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) is defined in the current lymphoma classifications as a T-cell non-Hodgkin's lymphoma. However, in approximately one third of the cases of this lymphoproliferative disease rearrangements of T-cell receptor (TCR) genes indicating clonal expansion of T cells are not detectable. It is currently believed that these cases may represent early stages of a lymphoma with a minor oligoclonal T-cell population. In the present study, 18 lymph nodes with the characteristic histology of AILD were investigated for clonal T-cell receptor gene rearrangements by analysis of DNA extracted from whole tissue sections. Dominant T-cell clones were detected in 12 of these cases. Single CD4(+) and CD8(+) T cells and proliferating Ki67(+) cells of seven cases were micromanipulated from frozen tissue sections. TCRbeta gene rearrangements were amplified from these cells by polymerase chain reaction and sequenced. In all informative cases, the clonal gene rearrangements were only detected among CD4(+), and not among CD8(+) T cells, indicating that the tumor clones in AILD usually derive from CD4(+) T cells. Minor clonal T-cell populations in those cases in which no clone was found by whole-tissu...Continue Reading

References

Jan 2, 1975·The New England Journal of Medicine·R J Lukes, B H Tindle
May 23, 1975·Deutsche medizinische Wochenschrift·T Radaszkiewicz, K Lennert
Feb 1, 1992·The Journal of Clinical Investigation·T SumidaS Yoshida
Jan 1, 1990·Annual Review of Biochemistry·K I AraiT Yokota
Jan 1, 1986·Acta Haematologica·R PirkerF Prischl
Nov 1, 1986·Journal of Clinical Pathology·N T O'ConnorD Y Mason
Jun 1, 1986·Annals of the Rheumatic Diseases·Y J BignonB J Sauvezie
Feb 1, 1987·The Journal of Clinical Investigation·E H LipfordJ Cossman
Jan 1, 1985·Virchows Archiv. A, Pathological Anatomy and Histopathology·H KnechtK Lennert
Jun 1, 1974·Lancet·G FrizzeraH Rappaport
Jun 1, 1981·American Journal of Clinical Pathology·L BorosJ P D'Souza
Mar 7, 2000·The American Journal of Pathology·A RoersR Küppers

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Citations

Oct 14, 2004·Virchows Archiv : an International Journal of Pathology·Klaus WillenbrockMartin-Leo Hansmann
Dec 7, 2007·The American Journal of Surgical Pathology·Yoshiko Idate MurakamiNaoyoshi Mori
Jul 10, 2007·International Journal of Laboratory Hematology·A J BenchM A Scott
Dec 8, 2009·British Journal of Haematology·Laurence de LevalPhilippe Gaulard
Sep 2, 2015·Nature Communications·Eliana RuggieroChristof von Kalle
Dec 1, 2007·Advances in Anatomic Pathology·Bharat N NathwaniMilton R Drachenberg
Nov 28, 2002·International Journal of Cancer. Journal International Du Cancer·Seung-Sook LeeHans Konrad Müller-Hermelink
Jul 22, 2004·The Oncologist·Jennifer R Brown, Arthur T Skarin
Jan 26, 2011·Histopathology·Laurence de Leval, Philippe Gaulard
Jan 24, 2015·Experimental & Molecular Medicine·Qingqing CaiKen H Young
Jun 5, 2003·British Journal of Haematology·Ahmet DoganChara Kyriakou
Nov 2, 2006·Archives of Pathology & Laboratory Medicine·Robert C HawleyRichard J Zarbo

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