Analysis of Temporal Changes in Growth and Gene Expression for Commensal Gut Microbes in Response to the Polyphenol Naringenin

Microbiology Insights
Jenni FirrmanWeidong Xiao

Abstract

In this study, the effect of the flavanone naringenin on the growth and genetic expression of the commensal gut microbes, Ruminococcus gauvreauii, Bifidobacterium catenulatum, and Enterococcus caccae, was analyzed. Analysis of growth curves revealed that Ruminococcus gauvreauii was unaffected by naringenin, Bifidobacterium catenulatum was slightly enhanced by naringenin, and Enterococcus caccae was severely inhibited by naringenin. Changes in genetic expression due to naringenin were determined using single-molecule RNA sequencing. Analysis revealed the following responses to naringenin: Ruminococcus gauvreauii upregulated genes involved in iron uptake; Bifidobacterium catenulatum upregulated genes involved in cellular metabolism, DNA repair and molecular transport, and downregulated genes involved in thymidine biosynthesis and metabolism; Enterococcus caccae upregulated pathways involved in transcription and protein transport and downregulated genes responsible for sugar transport and purine synthesis. For the first time, changes in growth and gene expression for commensal gut bacteria in response to naringenin were documented.

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Citations

Apr 1, 2021·The Journal of Nutritional Biochemistry·Tori ShockKristina Martinez-Guryn
Jul 17, 2021·Frontiers in Nutrition·Maria Carolina Rodríguez-DazaDenis Roy
Aug 28, 2021·Cancers·Raghad Khalid Al-IshaqDietrich Büsselberg

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Methods Mentioned

BETA
phosphotransferase
glycosylation

Software Mentioned

SeqLL
UCLUST

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