PMID: 8954163Nov 15, 1996Paper

Analysis of the 1H-NMR chemical shifts of Cu(I)-, Cu(II)- and Cd-substituted pea plastocyanin. Metal-dependent differences in the hydrogen-bond network around the copper site

European Journal of Biochemistry
M UbbinkD S Bendall

Abstract

To compare cadmium-substituted plastocyanin with copper plastocyanin, the 1H-NMR spectra of CuI-, CuII- and Cd-plastocyanin from pea have been analyzed. Full assignments of the spectra of CuI- and Cd-plastocyanin indicate chemical shift differences up to 1 ppm. The affected protons are located in the four loops that surround the Cu site. The largest differences were found for protons in the hydrogen bond network which stabilizes this part of the protein. This suggests that the chemical shift differences are caused by very small but extensive structural changes in the network upon replacement of CuI by Cd. For CuII-plastocyanin the resonances of 72% of the protons observed in the CuI form have been identified. Protons within approximately 0.9 nm of the CuII were not observed due to fast paramagnetic relaxation. The protons between 0.9-1.7 nm from the CuII showed chemical shift differences up to 0.4 ppm compared to both CuI- and Cd-plastocyanin. These differences can be predicted assuming that they represent pseudocontact shifts. When corrected for the pseudocontact shift contribution, the CuII-plastocyanin chemical shifts were nearly all identical within error to those of the Cd form, but not of the CuI-plastocyanin, indicating ...Continue Reading

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Citations

Jul 12, 2001·Chemistry : a European Journal·I M van AmsterdamG W Canters
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·J M NocekB M Hoffman
May 3, 2005·Biochimica Et Biophysica Acta·Christian LangeMarcellus Ubbink
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Feb 16, 2006·Magnetic Resonance in Chemistry : MRC·Malene Ringkjøbing JensenJens J Led
Nov 8, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lars Elkjaer JørgensenEva Danielsen
Feb 26, 2013·Magnetic Resonance in Chemistry : MRC·Abdul-Hamid M EmwasHarbi T Al-Masri
May 24, 2012·Chembiochem : a European Journal of Chemical Biology·Sandra ScanuMarcellus Ubbink
May 23, 2000·Biochemistry·M EjdebäckM Ubbink
Oct 25, 2001·Journal of the American Chemical Society·P B CrowleyM Ubbink
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Surya B AdhikariGuijun Wang
Sep 30, 2010·Journal of the American Chemical Society·Francesca MeschiMarcellus Ubbink
Jun 11, 2019·The Journal of Physical Chemistry Letters·Linda CerofoliniClaudio Luchinat
Aug 31, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Israel AlshanskiMattan Hurevich
Mar 21, 2007·Journal of the American Chemical Society·Rinske HulskerMarcellus Ubbink
Jan 19, 2008·Journal of the American Chemical Society·Rinske HulskerMarcellus Ubbink

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