PMID: 8995704Feb 1, 1997Paper

Analysis of the 2-kilobase latency-associated transcript expressed in PC12 cells productively infected with herpes simplex virus type 1: evidence for a stable, nonlinear structure

Journal of Virology
E Rødahl, L Haarr

Abstract

The major latency-associated transcript (LAT) expressed in PC12 cells productively infected with herpes simplex virus type 1 is a 2-kb, nonpolyadenylated RNA molecule that accumulates in the nuclei of infected cells. In actinomycin D-treated cells, the 2-kb LAT gene transcript has a half-life considerably greater than 12 h. After polyacrylamide gel electrophoresis, two species of the transcript were observed, a major species that was retarded in the gel and a minor species that migrated as a 1.96-kb RNA molecule. RNase H digestion after hybridization of the RNA with an oligonucleotide complementary to positions -80 to -101 relative to the 3' end of the 2-kb LAT gene transcript changed the mobility of the retarded species into that of the rapidly migrating species. Our data indicate that the 2-kb LAT gene transcript expressed in productively infected PC12 cells is present in a stable, nonlinear form.

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Citations

Jul 25, 2014·Journal of Virology·Toni M Schwarz, Caroline A Kulesza
Dec 14, 2011·FEMS Microbiology Reviews·Michael P NicollStacey Efstathiou
Jan 21, 2000·The Journal of General Virology·C M Preston
Feb 8, 2003·DNA and Cell Biology·Edward A BurtonJoseph C Glorioso
Jan 22, 2013·Journal of Neurovirology·Kerry K BrinkmanNigel W Fraser
Jul 25, 2013·Wiley Interdisciplinary Reviews. RNA·Jay R Hesselberth
Jul 1, 1997·Clinical Microbiology Reviews·E K Wagner, D C Bloom
Mar 21, 2015·Genes & Development·Kazimierz T TycowskiJoan A Steitz
Jul 24, 2014·RNA·Gaëlle J S Talhouarne, Joseph G Gall

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