PMID: 7894747Aug 1, 1994

Analysis of the Anopheles gambiae genome using RAPD markers

Insect Molecular Biology
G FaviaC Louis

Abstract

RAPD analysis technique is used as a rapid and reliable tool for genome analysis in the malaria vector Anopheles gambiae. Using more than eighty different commercially available primers we identified more than sixty different DNA segments that were differentially amplified in different strains of An. gambiae s.s. and An. arabiensis. An estimate of the cytogenetic position of these markers is provided by their hybridization to divisional dot-blot filters. Potentially useful RAPD markers can be cytogenetically mapped with more precision by in situ hybridization and, as they segregate as dominant markers in a Mendelian fashion, they can also be genetically mapped relative to other genes or rearrangements. Finally, we identified markers for their potential use in the identification of different mosquito strains.

References

Jul 14, 2005·Genetica·Lilian Castiglioni, Hermione Elly Melara de Campos Bicudo
Nov 19, 2002·International Journal for Parasitology·Douglas E Norris
Feb 17, 2000·International Journal for Parasitology·N W Beebe, R D Cooper
Oct 1, 1996·Trends in Genetics : TIG·G FaviaA della Torre
Feb 6, 2007·Parasitology·P Willadsen
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·F CatterucciaT G Loukeris
Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tapan K BeraIra Pastan
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dana ThomasovaFrank H Collins
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tapan K BeraIra Pastan
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·F OduolK D Vernick
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·J VizioliGeorge Dimopoulos
Dec 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tapan K BeraIra Pastan
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·G FaviaC Louis
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·George DimopoulosC Louis
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·K D MathiopoulosM Coluzzi
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·B ArcáM Coluzzi
Jan 10, 2003·Molecular Biology and Evolution·Vladimir V Kapitonov, Jerzy Jurka
Dec 9, 2000·Annual Review of Entomology·D W SeversonD L Knudson
Apr 1, 2009·PLoS Neglected Tropical Diseases·Barry J BeatyFotis C Kafatos
Oct 13, 2009·Expert Review of Molecular Diagnostics·Shuzhen SimGeorge Dimopoulos
Sep 14, 2006·Molecular Ecology·Susanta K Behura
Apr 13, 2000·Insect Molecular Biology·L SpanosC Louis
Sep 5, 2009·Applied Microbiology and Biotechnology·Bin CaoKai-Chee Loh
May 8, 1998·Nature·R Hengstenberg

Citations

May 1, 1992·Molecular Ecology·H HadrysB Schierwater
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L ZhengFotis C Kafatos
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Feb 25, 1991·Nucleic Acids Research·J Welsh, M McClelland
Dec 25, 1990·Nucleic Acids Research·J Welsh, M McClelland
Jan 1, 1993·Insect Molecular Biology·W C Black
Apr 1, 1992·Parasitology Today·M Coluzzi

Related Concepts

Cytogenetics
Genome
Nucleic Acid Hybridization Procedure
Genetic Markers
Genome Mapping
Malaria
Malaria Vaccines
Anopheles gambiae
Chromosome Inversion
Genetic Polymorphism

Related Feeds

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.