Analysis of the antibody response to an immunodominant epitope of the envelope glycoprotein of a lentivirus and its diagnostic potential

Journal of Clinical Microbiology
Franca MordasiniGiuseppe Bertoni

Abstract

The envelope glycoprotein of small ruminant lentiviruses (SRLV) is a major target of the humoral immune response and contains several linear B-cell epitopes. We amplified and sequenced the genomic segment encoding the SU5 antigenic site of the envelope glycoprotein of several SRLV field isolates. With synthetic peptides based on the deduced amino acid sequences of SU5 in an enzyme-linked immunosorbent assay (ELISA), we have (i) proved the immunodominance of this region regardless of its high variability, (ii) defined the epitopes encompassed by SU5, (iii) illustrated the rapid and peculiar kinetics of seroconversion to this antigenic site, and (iv) shown the rapid and strong maturation of the avidity of the anti-SU5 antibody. Finally, we demonstrated the modular diagnostic potential of SU5 peptides. Under Swiss field conditions, the SU5 ELISA was shown to detect the majority of infected animals and, when applied in a molecular epidemiological context, to permit rapid phylogenetic classification of the infecting virus.

References

Jul 1, 1987·The Veterinary Quarterly·E J van der Molen, D J Houwers
Dec 1, 1994·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·R G ZanoniE Peterhans
Aug 1, 1993·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·G RuffS Lazary
May 4, 1999·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·J C PlantierF Barin
Jun 10, 2003·Journal of Medical Virology·Peter RobertsonAndrew Lloyd
Mar 30, 2005·Veterinary Microbiology·D de AndrésG D Harkiss
Jun 9, 2005·Clinical and Diagnostic Laboratory Immunology·Lynn M HerrmannDonald P Knowles

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Citations

Jul 26, 2011·Veterinary Research·Karlen GazarianSebastian Aguilar Pierle
Jan 28, 2012·BMC Veterinary Research·Hugo RamírezDamián de Andrés
Apr 25, 2013·Viruses·Hugo RamírezHumberto A Martínez
Feb 5, 2013·Veterinary Immunology and Immunopathology·X de AndrésB Amorena
Dec 3, 2010·Veterinary Immunology and Immunopathology·Ramses ReinaSergio Rosati
Nov 7, 2008·Veterinary Microbiology·Britt GjersetChristine Monceyron Jonassen
Dec 1, 2012·The Journal of General Virology·Antoine RachidStephen Valas
Apr 4, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Elizabeth J B ManningCynthia B Wolf
Jun 20, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Stefano NardelliAlexander Tavella
Mar 2, 2021·PLoS Computational Biology·Matthew I J RaybouldCharlotte M Deane

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