Analysis of the antimicrobial and anti-caries effects of TiF4 varnish under microcosm biofilm formed on enamel

Journal of Applied Oral Science : Revista FOB
Beatriz Martines de SouzaAna Carolina Magalhães

Abstract

Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect. This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model. A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. All experiments were performed in biological triplicate (n=4/group in each experiment). Factors evaluated were: bacterial viability (% dead and live bacteria); CFU counting (log10 CFU/mL); and enamel demineralization (transverse microradiography - TMR). Data were analysed using ANOVA/Tukey's test or Kruskal-Wallis/Dunn's test (p<0.05). Only chlorhexidine significantly increased the number of dead bacteria (68.8±13.1% dead bacteria) compared to untreated control (48.9±16.1% dead bacteria). No treatment reduced the CFU counting (total microorganism and total streptococci) compared to the negative controls. Only TiF4 was able to reduce enamel de...Continue Reading

References

Jun 1, 1989·Oral Microbiology and Immunology·G H Bowden, I R Hamilton
Feb 18, 2003·Oral Microbiology and Immunology·J PrattenD A Spratt
Sep 5, 2009·Advances in Applied Microbiology·Andrew J McBain
Sep 18, 2009·European Journal of Oral Sciences·Juliana P M LimaIriana C J Zanin
Dec 21, 2010·Evidence-based Dentistry·Paul Ashley
Jan 19, 2011·Archives of Oral Biology·Santosh PanditJae-Gyu Jeon
Sep 24, 2011·Journal of Dental Research·N Takahashi, J Washio
Aug 29, 2012·Journal of Applied Microbiology·J D RudneyC Aparicio
Dec 5, 2012·Caries Research·B NyvadD Beighton
Sep 18, 2015·Journal of Dental Research·N Takahashi
Feb 27, 2016·Caries Research·Cácia SignoriMaximiliano S Cenci

❮ Previous
Next ❯

Citations

Jan 10, 2019·Journal of Applied Oral Science : Revista FOB·Daiana Moreli Soares Dos SantosAna Carolina Magalhães
Dec 23, 2020·Biofouling·Débora de Souza Collares Maia Castelo-BrancoJosé Júlio Costa Sidrim
Feb 17, 2021·Biofouling·Tamara Teodoro AraujoMarília Afonso Rabelo Buzalaf
Mar 18, 2021·Scientific Reports·Beatriz Martines de SouzaAna Carolina Magalhães
Jun 27, 2021·Journal of Dentistry·Mariele VertuanAna Carolina Magalhães
May 16, 2019·Caries Research·Daiana Moreli Soares Dos SantosAna Carolina Magalhães

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence

Software Mentioned

Leica Application Suite - Advanced Fluorescence
BioImage L
GraphPad
Graph Pad Instat
Mahr Surf XCR
Inspektor Research System

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.