Analysis of the Candida albicans Phosphoproteome

Eukaryotic Cell
Sven D WillgerDeborah A Hogan

Abstract

Candida albicans is an important human fungal pathogen in both immunocompetent and immunocompromised individuals. C. albicans regulation has been studied in many contexts, including morphological transitions, mating competence, biofilm formation, stress resistance, and cell wall synthesis. Analysis of kinase- and phosphatase-deficient mutants has made it clear that protein phosphorylation plays an important role in the regulation of these pathways. In this study, to further our understanding of phosphorylation in C. albicans regulation, we performed a deep analysis of the phosphoproteome in C. albicans. We identified 19,590 unique peptides that corresponded to 15,906 unique phosphosites on 2,896 proteins. The ratios of serine, threonine, and tyrosine phosphosites were 80.01%, 18.11%, and 1.81%, respectively. The majority of proteins (2,111) contained at least two detected phosphorylation sites. Consistent with findings in other fungi, cytoskeletal proteins were among the most highly phosphorylated proteins, and there were differences in Gene Ontology (GO) terms for proteins with serine and threonine versus tyrosine phosphorylation sites. This large-scale analysis identified phosphosites in protein components of Mediator, an imp...Continue Reading

References

Nov 1, 1996·Molecular Biology of the Cell·R A SiaD J Lew
Jan 1, 1997·Progress in Cell Cycle Research·A Fattaey, R N Booher
Oct 21, 1998·Oncogene·N Dhanasekaran, E Premkumar Reddy
Jan 29, 2000·Molecular Microbiology·A SonnebornJ F Ernst
Jul 4, 2001·Trends in Microbiology·R A Calderone, W A Fonzi
Aug 28, 2003·Molecular Microbiology·Xin-De ZhengYue Wang
Jan 22, 2004·FEMS Yeast Research·Michael KruppaRichard Calderone
Feb 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Magnus HallbergStefan Björklund
Aug 13, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hilmar WisplinghoffMichael B Edmond
Jan 22, 2005·Molecular Microbiology·Takashi UmeyamaYoshimasa Uehara
Aug 17, 2005·PLoS Genetics·Burkhard R BraunAndré Nantel
Sep 13, 2005·Annual Review of Microbiology·Carol A Kumamoto, Marcelo D Vinces
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yelena V BudovskayaPaul K Herman
Mar 22, 2006·Microbiology·R Alonso MongeJ Pla
Jan 16, 2007·Clinical Microbiology Reviews·M A Pfaller, D J Diekema
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Judit VillénSteven P Gygi
Feb 9, 2008·Journal of Proteome Research·Joshua T Wilson-GradySteven P Gygi
Oct 16, 2008·Clinical Microbiology Reviews·David TrofaJoshua D Nosanchuk
Mar 21, 2009·Nature Protocols·Paul J BoersemaAlbert J R Heck
Dec 10, 2009·Future Microbiology·Deborah A Hogan, Paula Sundstrom
Jan 12, 2010·Analytical and Bioanalytical Chemistry·Ulli Martin HohenesterSimone König
Feb 9, 2010·PLoS Pathogens·Jill R BlankenshipAaron P Mitchell
Jun 15, 2010·Current Opinion in Microbiology·Dongmei LiRichard Calderone
Feb 8, 2011·PLoS Computational Biology·Nozomu YachieYasushi Ishihama
Jul 14, 2011·Proteomics·Benoît ValotMichel Zivy
Sep 20, 2011·Molecular Microbiology·Theresia LassakJoachim F Ernst
Nov 15, 2011·Journal of Proteome Research·Thomas TausKarl Mechtler
Jan 31, 2012·Molecular & Cellular Proteomics : MCP·Grigoris D AmoutziasStephen G Oliver

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Citations

Dec 27, 2015·Medical Mycology·Mohammad T Albataineh, David Kadosh
Mar 24, 2016·Journal of Proteomics·Eva LiñeiroFrancisco Javier Fernández-Acero
Sep 9, 2016·Journal of Proteome Research·Hailin ZhengWeida Liu
Sep 24, 2016·Future Microbiology·Regina Helena PiresMaria José Soares Mendes-Giannini
Aug 15, 2018·Omics : a Journal of Integrative Biology·Arun H PatilThottethodi Subrahmanya Keshava Prasad
Dec 28, 2019·Journal of Fungi·Kyunghun MinJames B Konopka
Feb 28, 2020·Journal of Fungi·Christiane RollenhagenSamuel A Lee
Jul 10, 2019·Scientific Reports·Almudena Escobar-NiñoFrancisco Javier Fernández-Acero
Mar 3, 2017·G3 : Genes - Genomes - Genetics·Panayotis VlastaridisGrigorios D Amoutzias
Mar 16, 2017·Fungal Genetics and Biology : FG & B·Liliane F C RibeiroMark R Marten
Jun 15, 2016·Journal of Proteome Research·Xiaowei ZhouWeida Liu
Sep 1, 2021·Molecular Microbiology·Carla E LanzeJames B Konopka

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