Analysis of the Catecholaminergic Phenotype in Human SH-SY5Y and BE(2)-M17 Neuroblastoma Cell Lines upon Differentiation

PloS One
Roberta FilogranaMarco Bisaglia

Abstract

Human cell lines are often used to investigate cellular pathways relevant for physiological or pathological processes or to evaluate cell toxicity or protection induced by different compounds, including potential drugs. In this study, we analyzed and compared the differentiating activities of three agents (retinoic acid, staurosporine and 12-O-tetradecanoylphorbol-13-acetate) on the human neuroblastoma SH-SY5Y and BE(2)-M17 cell lines; the first cell line is largely used in the field of neuroscience, while the second is still poorly characterized. After evaluating their effects in terms of cell proliferation and morphology, we investigated their catecholaminergic properties by assessing the expression profiles of the major genes involved in catecholamine synthesis and storage and the cellular concentrations of the neurotransmitters dopamine and noradrenaline. Our results demonstrate that the two cell lines possess similar abilities to differentiate and acquire a neuron-like morphology. The most evident effects in SH-SY5Y cells were observed in the presence of staurosporine, while in BE(2)-M17 cells, retinoic acid induced the strongest effects. Undifferentiated SH-SY5Y and BE(2)-M17 cells are characterized by the production of b...Continue Reading

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Citations

Aug 9, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Bartosz PomiernyBogusława Budziszewska
Jan 26, 2017·Molecular Neurodegeneration·Helena XicoyGerard J M Martens
Jun 3, 2016·Physiological Reports·Francesca UbertiClaudio Molinari
Apr 23, 2020·Journal of Neurochemistry·Igor C FontanaEduardo R Zimmer
Sep 7, 2019·Molecular Neurodegeneration·Anna MasatoLuigi Bubacco
Jul 10, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ikumi KitazonoAkihide Tanimoto
Jan 9, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Rui F SimõesTeresa Cunha-Oliveira
Jan 12, 2021·Frontiers in Cellular Neuroscience·Shuheng WenKoichi Uemura
May 1, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Prathyushakrishna MachaMilana C Vasudev
Jul 31, 2021·Journal of Hazardous Materials·Katherine R LandwehrUNKNOWN WAERP
Sep 8, 2021·Frontiers in Chemistry·Mathias BrouillardValérie Desvergnes

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Methods Mentioned

BETA
light microscopy
PCR
protein assay
electrophoresis

Software Mentioned

GraphPad Prism

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