Analysis of the cellular expression pattern of beta-CGRP in alpha-CGRP-deficient mice

The Journal of Comparative Neurology
Burkhard SchützAndreas Zimmer

Abstract

In this study we compared the alpha-calcitonin gene-related peptide (alphaCGRP) and betaCGRP expression patterns in wild-type and knockout mice by using quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry. In dorsal root ganglia and spinal cord of wild-type animals, alphaCGRP mRNA was about two times more abundant than betaCGRP mRNA. The betaCGRP mRNA was the only isoform expressed in the intestine. In alphaCGRP knockout mice, we found no change in betaCGRP mRNA levels in dorsal root ganglia and spinal cord compared with wild-type controls, but a twofold decrease in the intestine. CGRP immunoreactivity (IR) was detected in many small and some large neurons in the dorsal root ganglia, was found in sensory fibers and motor neurons in the spinal cord, and labeled neuromuscular junctions in wild-type mice. In the dorsal root ganglia of alphaCGRP knockout mice, punctate betaCGRP-IR again was predominantly found in small neurons. In the spinal cord, betaCGRP-IR fibers were localized to the outermost layer of the dorsal horn. IR was found in the cell bodies of motor neurons, but it was undetectable in neuromuscular junctions. In the intestine, CGRP-IR was localized to neurons of the myenteric plexus a...Continue Reading

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Citations

Sep 15, 2005·Cell and Tissue Research·Célia Ribeiro DuarteAndreas Zimmer
May 30, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Joeri Van Op den boschJean-Pierre Timmermans
Dec 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Isabel DevesaAntonio Ferrer-Montiel
Mar 11, 2015·Current Pain and Headache Reports·Sarah Walter, Marcelo E Bigal
Sep 23, 2014·Cellular and Molecular Neurobiology·Burkhard SchützEberhard Weihe
May 15, 2007·Behavioural Brain Research·De-Yong LiangJ David Clark
Mar 23, 2017·The Journal of Comparative Neurology·Christine M BarryRainer V Haberberger
Nov 21, 2016·Neuropeptides·Eric A KaiserAndrew F Russo
Mar 24, 2018·Cephalalgia : an International Journal of Headache·Erica R HendrikseChristopher S Walker
Nov 9, 2017·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Hsiangkuo YuanStephen D Silberstein
Nov 20, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Nick J SpencerTim Hibberd
Apr 26, 2006·Journal of Vascular Research·Chandra SomasundaramRichard D Bukoski
Mar 6, 2007·Journal of Neuroscience Research·Mendell Rimer
Nov 3, 2009·The Journal of Comparative Neurology·Laurent GautronJoel Elmquist
Jan 17, 2019·Clinical Pharmacology and Therapeutics·Hsiangkuo YuanStephen D Silberstein
Oct 8, 2014·Physiological Reviews·F A RussellS D Brain
Nov 4, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Nick J SpencerTim Hibberd
Aug 3, 2018·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Shuta MishimaNarumi Hashikawa
Oct 24, 2017·British Journal of Pharmacology·Debbie L HayChristopher S Walker
Jun 30, 2021·British Journal of Pharmacology·Tayla A ReesChristopher S Walker

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