Analysis of the cellulose synthase operon genes, bcsA, bcsB, and bcsC in Cronobacter species: Prevalence among species and their roles in biofilm formation and cell-cell aggregation

Food Microbiology
Lan HuB D Tall

Abstract

Cronobacter species are emerging food-borne pathogens that cause severe sepsis, meningitis, and necrotizing entercolitis in neonates and infants. Bacterial pathogens such as Escherichia coli and Salmonella species produce extracellular cellulose which has been shown to be involved in rugosity, biofilm formation, and host colonization. In this study the distribution and prevalence of cellulose synthase operon genes (bcsABZC) were determined by polymerase chain reaction (PCR) analysis in 231 Cronobacter strains isolated from clinical, food, environmental, and unknown sources. Furthermore, bcsA and bcsB isogenic mutants were constructed in Cronobacter sakazakii BAA894 to determine their roles. In calcofluor binding assays bcsA and bcsB mutants did not produce cellulose, and their colonial morphotypes were different to that of the parent strain. Biofilm formation and bacterial cell-cell aggregation were significantly reduced in bcsA and bcsB mutants compared to the parental strain. bcsA or bcsAB PCR-negative strains of C. sakazakii did not bind calcofluor, and produced less biofilm and cell-cell aggregation compared to strains possessing bcsAB genes. These data indicated that Cronobacter bcsABZC were present in all clinical isolate...Continue Reading

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Citations

Jun 18, 2016·TheScientificWorldJournal·Samaneh Sadat MalekiKong-Shu Ji
Jan 18, 2018·Scientific Reports·Seongok KimSangryeol Ryu
Mar 16, 2017·Frontiers in Microbiology·Jennifer L Hoeflinger, Michael J Miller
Jul 25, 2019·Frontiers in Microbiology·Mohamed A AlyErik Reimhult
Jul 15, 2017·Journal of Phycology·Anchittha Satjarak, Linda E Graham
Dec 18, 2020·Frontiers in Microbiology·Yixiao HuangChao Shi
Nov 7, 2020·Microbial Pathogenesis·Esteban-Kenel VeronicaCruz-Córdova Ariadnna

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