Analysis of the ciprofloxacin-induced mutations in Salmonella typhimurium

Environmental and Molecular Mutagenesis
B ClerchM Llagostera

Abstract

The mutagenic events induced by ciprofloxacin, a potent antimicrobial agent, have been characterized. For this, a battery of His mutants of Salmonella typhimurium (hisG428, his G46, His C9070, and his G1775 targets) that detects the six possible transitions and transversions [Levin and Ames (1986): Environ Mutagen 8:9-28] and two additional His strains (hisC3076 and his D3052 targets) carrying frameshift mutations have been used. Our results indicate that GC-TA transversions are the major base-pair substitution induced by ciprofloxacin and that GC-At transitions are also produced, but to a lesser degree. However, we cannot discard the fact that At-Ta transversions are also induced. In addition, the data indicate that the mutational specificity of ciprofloxacin depends on the location of the target. Intragenic base-pair substitutions are the most frequent mutations at the hisG428 target when it is on the chromosome, whereas 3 or 6 base-pair deletions are the major mutagenic events when this target is on the plasmid pAQ1. We have shown that ciprofloxacin also induces deletions/insertions at the hisC3076 and hisD3052 frameshift targets. Therefore, this inhibitor of DNA gyrase promotes a wide pattern of mutations including differen...Continue Reading

References

Jan 1, 1979·Journal of Bacteriology·F WinstonJ H Miller
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L J Gudas, A B Pardee
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·R J Reece, A Maxwell
May 1, 1991·Mutation Research·E Gocke
Nov 1, 1987·The Journal of Antimicrobial Chemotherapy·I PhillipsF Baquero
Jan 1, 1986·Environmental Mutagenesis·P E HartmanD E Levin
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B N AmesW E Durston
May 1, 1983·Mutation Research·D M Maron, B N Ames
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D E LevinB N Ames
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D E LevinB N Ames
Jan 1, 1994·Environmental and Molecular Mutagenesis·J G LevineD M DeMarini
Jan 1, 1994·Environmental and Molecular Mutagenesis·E KupchellaT A Cebula
Apr 1, 1993·Antimicrobial Agents and Chemotherapy·H YoshidaS Nakamura

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